Protein phosphorylation mediated by IL-2/IL-2 receptor β-chain interaction

T. Ishii, T. Takeshita, N. Numata, K. Sugamura

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36 Citations (Scopus)

Abstract

We previously found that IL-2 rapidly induced protein phosphorylation of a 67-kDa (pp67) and four 63-kDa (pp63s) cellular proteins in various T cells. Here, we show that the IL-2-stimulated phosphorylation is mediated by the IL-2R-β chain composed of the high affinity IL-2R, and induced by activation of Ca2+/phospholipid-dependent protein kinase C (PKC). The IL-2-stimulated phosphorylation was always observed in various T cell lines bearing high affinity IL-2R, but never observed in cells which express only low affinity IL-2R consisted of α-chain alone. When the expression of high affinity IL-2R was modified by anti-IL-2R mAb for reducing the affinity to 8- to 10-fold lower without affecting the sites of IL-2R, the effective dose of IL-2 on phosphorylation of pp67 increased 8 to 10 times. When cells were treated with pronase, approximately 95% sites of low affinity IL-2R were selectively decreased, but the IL-2 dose dependency for pp67 phosphorylation was little affected. These data exactly suggest that protein phosphorylation in response to IL-2 such as pp67 and pp63s, is mediated by high affinity but not low affinity IL-2R. Furthermore, the IL-2-stimulated phosphorylation of these proteins was also observed in MLA 144 cells which express only low affinity IL-2R consisting of β-chain alone. In addition, various phorbol esters and tumor promoters, which activate PKC, were also demonstrated to induce the phosphorylation of a pp67 and pp63s in these T cell lines. Therefore, the present study suggests that IL-2/IL-2R β-chain interaction triggers the phosphorylation of pp67 and pp63s, where the PKC may have an important role.

Original languageEnglish
Pages (from-to)174-179
Number of pages6
JournalJournal of Immunology
Volume141
Issue number1
Publication statusPublished - 1988 Jan 1

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Ishii, T., Takeshita, T., Numata, N., & Sugamura, K. (1988). Protein phosphorylation mediated by IL-2/IL-2 receptor β-chain interaction. Journal of Immunology, 141(1), 174-179.