Protein ligand-tethered synthetic calcium indicator for localization control and spatiotemporal calcium imaging in plant cells

Yousuke Takaoka, Miyuki Shigenaga, Masaki Imai, Yuuki Nukadzuka, Yasuhiro Ishimaru, Kei Saito, Ryusuke Yokoyama, Kazuhiko Nishitani, Minoru Ueda

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

In plant biology, calcium ions are involved in a variety of intriguing biological phenomena as a secondary messenger. However, most conventional calcium indicators are not applicable for plant cells because of the difficulty with their localization control in plant cells. We here introduce a method to monitor spatiotemporal Ca 2+ dynamics in living plant cells based on linking the synthetic calcium indicator Calcium Green-1 to a natural product-based protein ligand. In a proof-of-concept study using cultured BY-2 cells overexpressing the target protein for the ligand, the ligand-tethered probe accumulated in the cytosol and nucleus, and enabled real-time monitoring of the cytosolic and nucleus Ca 2+ dynamics under the physiological condition. The present strategy using ligand-tethered fluorescent sensors may be successfully applied to reveal the spatiotemporal dynamics of calcium ions in living plant cells.

Original languageEnglish
Pages (from-to)9-14
Number of pages6
JournalBioorganic and Medicinal Chemistry Letters
Volume26
Issue number1
DOIs
Publication statusPublished - 2016 Jan 1

Keywords

  • Calcium indicator
  • Chemical biology
  • Natural products
  • Plant cell

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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