Protein kinase B (PKB/Akt) is required for the completion of meiosis in mouse oocytes

Yumi Hoshino, Eimei Sato

    Research output: Contribution to journalArticle

    48 Citations (Scopus)

    Abstract

    Akt, also known as protein kinase B, is implicated in many cellular processes. Akt is phosphorylated at two residues, Thr308 and Ser473. Thr308-phosphorylated Akt is present in pericentriolar materials, while localization of Ser473-phosphorylated Akt was similar to that of microtubules in metaphase oocytes. Spindles were shorter and aberrant in oocytes injected with Thr308- or Ser473-phosphorylated Akt antibodies. Specifically, Thr308- and Ser473-phosphorylated Akts function individually and are both necessary to assemble the metaphase II (MII) spindle. Moreover, the functions of Thr308- and Ser473-phosphorylated Akts differ in MII oocytes. Although oocytes exhibited second polar body (PB2) emission after the injection of a peptide for Thr308, the chromosomal alignment and microtubular organization were aberrant. In contrast, the injection of a peptide for Ser473 caused a failure of PB2 emission. These results suggest that Thr308- and Ser473-phosphorylated Akts are individually involved in fertilization to complete meiosis, including different roles (i.e., Ser473-phosphorylated Akts are involved in PB2 emission, whereas Thr308-phosphorylated Akts regulate the organization of microtubules).

    Original languageEnglish
    Pages (from-to)215-223
    Number of pages9
    JournalDevelopmental Biology
    Volume314
    Issue number1
    DOIs
    Publication statusPublished - 2008 Feb 1

    Keywords

    • Akt/protein kinase B
    • Fertilization
    • Meiotic maturation
    • Microinjection
    • Mouse oocyte
    • Peptide
    • Polar body emission
    • SH-6/Akt inhibitor III
    • Spindle

    ASJC Scopus subject areas

    • Molecular Biology
    • Developmental Biology
    • Cell Biology

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