Protein-free culture of esophageal cancer cell lines

Y. Shimada, N. Yamaguchi, T. Akiyama, M. Fukumoto, T. Wagata, Y. Kanda, M. Imamura, T. Tobe

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


We have established 13 esophageal cancer cell lines capable of growing in a protein-free environment. The growth of these cells was not affected by conditioned medium, but the growth of NIH3T3 cells and human fibroblasts was stimulated by conditioned medium. On the other hand, conditioned medium inhibited the growth of human endothelial cells. Amplified int-2 oncogene correlated well with the growth of cells in a protein-free environment but the number of EGF receptors and growth effect of EGF did not relate to such growth. Esophageal cancer cells grow automatically, possibly involving mesenchymal cells via the paracrine system. This results in a poor prognosis in patients.

Original languageEnglish
Pages (from-to)315-320
Number of pages6
JournalHuman cell : official journal of Human Cell Research Society
Issue number4
Publication statusPublished - 1991 Dec

ASJC Scopus subject areas

  • Cell Biology
  • Cancer Research


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