Protein antiadsorption modification by negatively charged phospholipid polymer for microsystem needing electroosmotic flow

Yan Xu, Madoka Takai, Tomohiro Konno, Kazuhiko Ishihara

Research output: Contribution to conferencePaperpeer-review

Abstract

Biointerface with cell-membrane-like structure was constructed on the quartz microfluidic chip to suppress the non-specific protein adsorption as well as to retain the electroosomotic flow (EOF) effect through one-step modification. The PMBSSi, a negatively charged phospholipid copolymer containing 2-methacryloyloxyethyl phosphorylcholine (MPC) units, was synthesized to introduce such cell-membrane-like segments as well as surface charges onto the silica based microchannels via chemical bonding of the silane coupling units in the copolymer with the substrates. At neutral pH, the interface constructed by PMBSSi modification retained a significant cathodal EOF. Although the PMBSSi possesses negative charges, the nonspecific adsorptions of several kinds of proteins were effectively suppressed.

Original languageEnglish
Pages215-217
Number of pages3
Publication statusPublished - 2006 Jan 1
Externally publishedYes
Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan
Duration: 2006 Nov 52006 Nov 9

Other

Other10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
Country/TerritoryJapan
CityTokyo
Period06/11/506/11/9

Keywords

  • Electroosmotic flow
  • Phospholipid polymer
  • Protein antiadsorption
  • Surface modification

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Fingerprint

Dive into the research topics of 'Protein antiadsorption modification by negatively charged phospholipid polymer for microsystem needing electroosmotic flow'. Together they form a unique fingerprint.

Cite this