Protein and Organic-Molecular Crystallography With 300kV Electrons on a Direct Electron Detector

Kiyofumi Takaba, Saori Maki-Yonekura, Satoru Inoue, Tatsuo Hasegawa, Koji Yonekura

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Electron 3D crystallography can reveal the atomic structure from undersized crystals of various samples owing to the strong scattering power of electrons. Here, a direct electron detector DE64 was tested for small and thin crystals of protein and an organic molecule using a JEOL CRYO ARM 300 electron microscope. The microscope is equipped with a cold-field emission gun operated at an accelerating voltage of 300 kV, quad condenser lenses for parallel illumination, an in-column energy filter, and a stable rotational goniometer stage. Rotational diffraction data were collected in an unsupervised manner from crystals of a heme-binding enzyme catalase and a representative organic semiconductor material Ph-BTBT-C10. The structures were determined by molecular replacement for catalase and by the direct method for Ph-BTBT-C10. The analyses demonstrate that the system works well for electron 3D crystallography of these molecules with less damaging, a smaller point spread, and less noise than using the conventional scintillator-coupled camera.

Original languageEnglish
Article number612226
JournalFrontiers in Molecular Biosciences
Volume7
DOIs
Publication statusPublished - 2021 Jan 6

Keywords

  • CRYO ARM
  • DE64
  • eEFD
  • electron 3D crystallography (3D ED/MicroED)
  • energy filter

ASJC Scopus subject areas

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Molecular Biology

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