Protective effects of interleukin-10 on diaphragm muscle in a septic animal model

Azusa Taneda, Chiyohiko Shindoh, Yuzuru Ohuchi, Kunio Shirato

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The effects of Interleukin (IL)-10 intravenous injection after endotoxin administration on diaphragm muscle were studied using Wistar rats. The animals were divided into two treatment groups: A saline + endotoxin group as control and an IL-10+endotoxin group. E. coli endotoxin (10 mg/kg) was injected intraperitoneally 5 minutes after saline or IL-10 (1250 U, 0.25 mg) injection. The force-frequency curves, twitch kinetics and fatigability were measured at 0 and 4 hours after endotoxin injection. In the saline+endotoxin group, the force-frequency curves and half relaxation time were significantly decreased at 4 hours (p < 0.001 and p < 0.05, respectively) compared to those at 0 hour. In the IL-10+endotoxin group, the decrement in the force- frequency curves by endotoxin was prevented at 4 hours compared to that at 0 hour. Nicotinamide adenine dinucleotide phosphate (NADPH) diaphorase histochemistry showed positive staining in the saline+endotoxin at 4 hours, but there was no significant staining at 0 or 4 hours in the IL-0+endotoxin group. These data suggest that IL-10 prevents the deterioration of contraction induced by endotoxin by inhibiting nitric oxide production in the diaphragm muscle.

Original languageEnglish
Pages (from-to)45-54
Number of pages10
JournalTohoku Journal of Experimental Medicine
Volume185
Issue number1
DOIs
Publication statusPublished - 1998 May

Keywords

  • Endotoxin
  • NADPH diaphorase
  • Nitric oxide

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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