Protective effect of a prime-boost strategy with plasmid DNA followed by recombinant adenovirus expressing TgAMA1 as vaccines against Toxoplasma gondii infection in mice

Longzheng Yu, Junya Yamagishi, Shoufa Zhang, Chunmei Jin, Gabriel Oluga Aboge, Houshuang Zhang, Guohong Zhang, Tetsuya Tanaka, Kozo Fujisaki, Yoshifumi Nishikawa, Xuenan Xuan

    Research output: Contribution to journalArticle

    22 Citations (Scopus)

    Abstract

    A heterologous prime-boost strategy with priming plasmid DNA followed by recombinant virus expressing relevant antigens is known to stimulate protective immunity against intracellular parasites. In this study, we have evaluated a heterologous prime-boost strategy for immunizing mice against Toxoplasma gondii infection. Our results revealed that the prime-boost strategy using both plasmid DNA and adenoviral vector encoding TgAMA1 may stimulate both humoral and Th1/Th2 cellular immune responses specific for TgAMA1. Moreover, C57BL/6 mice immunized with the pAMA1/Ad5Null, pNull/Ad5AMA1, and pAMA1/Ad5AMA1 constructs showed survival rates of 12.5%, 37.5%, and 50%, respectively. In contrast, all the pNull/Ad5Null immunized mice died after infection with the PLK-GFP strain of T. gondii. Brain cyst burden was reduced by 23% in mice immunized with pAMA1/Ad5AMA1 compared with the pNull/Ad5AMA1 immunized mice. These results demonstrate that the heterologous DNA priming and recombinant adenovirus boost strategy may provide protective immunity against T. gondii infection.

    Original languageEnglish
    Pages (from-to)481-486
    Number of pages6
    JournalParasitology International
    Volume61
    Issue number3
    DOIs
    Publication statusPublished - 2012 Sep 1

    Keywords

    • Adenovirus
    • DNA vaccine
    • Heterologous prime-boost
    • TgAMA1
    • Toxoplasma gondii

    ASJC Scopus subject areas

    • Parasitology
    • Infectious Diseases

    Fingerprint Dive into the research topics of 'Protective effect of a prime-boost strategy with plasmid DNA followed by recombinant adenovirus expressing TgAMA1 as vaccines against Toxoplasma gondii infection in mice'. Together they form a unique fingerprint.

  • Cite this