Proteasome dysfunction induces muscle growth defects and protein aggregation

Yasuo Kitajima, Yoshitaka Tashiro, Naoki Suzuki, Hitoshi Warita, Masaaki Kato, Maki Tateyama, Risa Ando, Rumiko Izumi, Maya Yamazaki, Manabu Abe, Kenji Sakimura, Hidefumi Ito, Makoto Urushitani, Ryoichi Nagatomi, Ryosuke Takahashi, Masashi Aoki

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

The ubiquitin-proteasome and autophagy-lysosome pathways are the two major routes of protein and organelle clearance. The role of the proteasome pathway in mammalian muscle has not been examined in vivo. In this study, we report that the muscle-specific deletion of a crucial proteasomal gene, Rpt3 (also known as Psmc4), resulted in profound muscle growth defects and a decrease in force production in mice. Specifically, developing muscles in conditional Rpt3-knockout animals showed dysregulated proteasomal activity. The autophagy pathway was upregulated, but the process of autophagosome formation was impaired. A microscopic analysis revealed the accumulation of basophilic inclusions and disorganization of the sarcomeres in young adult mice. Our results suggest that appropriate proteasomal activity is important for muscle growth and for maintaining myofiber integrity in collaboration with autophagy pathways. The deletion of a component of the proteasome complex contributed to myofiber degeneration and weakness in muscle disorders that are characterized by the accumulation of abnormal inclusions.

Original languageEnglish
Pages (from-to)5204-5217
Number of pages14
JournalJournal of cell science
Volume127
Issue number24
DOIs
Publication statusPublished - 2014

Keywords

  • Autophagy
  • Muscle atrophy
  • Proteasome
  • Skeletal muscle

ASJC Scopus subject areas

  • Cell Biology

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