Protamine increases transfection efficiency and cell viability after transfection with calcium phosphate nanoparticles

Taichi Tenkumo, Olga Rotan, Viktoriya Sokolova, Matthias Epple

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Penetration through the cell membrane, endosomal escape and nuclear entry are the main barriers that transfection agents have to overcome. We prepared multi-shell calcium phosphate nanoparticles that were functionalized with DNA, polyethyleneimine (PEI) and protamine in order to improve transfection efficiency. PEI-functionalized calcium phosphate nanoparticles showed a high transfection efficiency, which was correlated with cytotoxity. Additional functinalization with protamine effectively reduced the cytotoxic effects of PEI, while maintaining the high transfection efficiency. Size,surface charge and morphology of multi-shell nanoparticles were analyzed by dynamic light scattering and scanning electron microscopy. The influence of nanoparticle concentration on the transfection efficiency and cell viability was tested on HeLa and MG-63 cell lines. Protamine-functinalized multi-shell calcium phosphate nanoparticles can serve as an efficient and non-toxic gene carrier for cell.

Original languageEnglish
Pages (from-to)64-74
Number of pages11
JournalNano Biomedicine
Volume5
Issue number2
Publication statusPublished - 2013 Jan 1

Keywords

  • Calcium phosphate
  • Cell viability
  • Fluorescence
  • Gene transfer
  • Nanoparticles

ASJC Scopus subject areas

  • Biomedical Engineering

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