Prostaglandin production by minced carrageenin granuloma tissue of rats and its inhibition by dexamethasone and cycloheximide

Kazuo Ohuchi, Hiroshi Sato, Tomoko Komabayashi, Susumu Tsurufuji, Hiroko Satoh, Lawrence Levine

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Seven-day-old carrageenin granuloma tissue of rats, when incubated in organ culture, produced 6-keto-prostaglandin F1(α), prostaglandin E2, prostaglandin F2(α), and thromboxane B2 in ratios of 16/7/1/1, respectively. Indomethacin (1 μg/ml) inhibited this cyclooxygenase-mediated arachidonic acid metabolism. Dexamethasone (1 μg/ml) also inhibited this metabolic cascade. Its inhibitory effect was not immediate; preincubation of the tissue with dexamethasone potentiated its inhibitory effectiveness. Cycloheximide (0.1 to 10 μg/ml) also inhibited prostaglandin production. The inhibitory mechanism by cycloheximide of prostaglandin production was discussed in relation to that by dexamethasone.

Original languageEnglish
Pages (from-to)267-274
Number of pages8
JournalProstaglandins and Medicine
Volume5
Issue number4
DOIs
Publication statusPublished - 1980 Jan 1

ASJC Scopus subject areas

  • Medicine(all)

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