Seven-day-old carrageenin granuloma tissue of rats, when incubated in organ culture, produced 6-keto-prostaglandin F1(α), prostaglandin E2, prostaglandin F2(α), and thromboxane B2 in ratios of 16/7/1/1, respectively. Indomethacin (1 μg/ml) inhibited this cyclooxygenase-mediated arachidonic acid metabolism. Dexamethasone (1 μg/ml) also inhibited this metabolic cascade. Its inhibitory effect was not immediate; preincubation of the tissue with dexamethasone potentiated its inhibitory effectiveness. Cycloheximide (0.1 to 10 μg/ml) also inhibited prostaglandin production. The inhibitory mechanism by cycloheximide of prostaglandin production was discussed in relation to that by dexamethasone.
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