Prostaglandin F regulates cytokine responses of mast cells through the receptors for prostaglandin E

Izumi Kaneko, Takanori Hishinuma, Kaori Suzuki, Yuji Owada, Noriko Kitanaka, Hisatake Kondo, Junichi Goto, Hiroshi Furukawa, Masao Ono

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

There is an increasing body of evidence that prostanoids modulate mast cell functions and contribute to the development of allergic inflammation. The present study aimed to identify an undetermined function of prostaglandin (PG) F in mast cell activation and the signaling mechanism involved in it. Simultaneous quantification of prostanoids by liquid chromatography/tandem mass spectrometry revealed the constitutive release of PGF, thromboxane B2, and 6-keto-PGF from bone marrow-derived mast cells (BMMCs). Upon activation of BMMCs by lipopolysaccharide, the cytokine production in BMMCs was enhanced when the culture was supplemented with PGF. However, F prostanoid receptor-a selective receptor for PGF-was not detected in BMMCs. Further investigations performed using prostanoid receptor antagonists revealed an alternative mechanism wherein the receptors for PGE species-E prostanoid receptors-mediated the PGF signal in BMMCs. The present study provides an insight into a novel function of PGF, i.e., an autocrine accelerator for mast cell activation.

Original languageEnglish
Pages (from-to)590-596
Number of pages7
JournalBiochemical and biophysical research communications
Volume367
Issue number3
DOIs
Publication statusPublished - 2008 Mar 14

Keywords

  • Autocrine
  • E prostanoid receptor
  • EP
  • Lipid mediator
  • Lipopolysaccharide
  • Mast cell
  • PGF

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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