TY - JOUR
T1 - Proposal of Helicobacter canicola sp. nov., previously identified as Helicobacter cinaedi, isolated from canines
AU - Kawamura, Yoshiaki
AU - Tomida, Junko
AU - Miyoshi-Akiyama, Tohru
AU - Okamoto, Tatsuya
AU - Narita, Masashi
AU - Hashimoto, Katsuhiko
AU - Cnockaert, Margo
AU - Vandamme, Peter
AU - Morita, Yuji
AU - Sawa, Tomohiro
AU - Akaike, Takaaki
N1 - Publisher Copyright:
© 2016 Elsevier GmbH
PY - 2016/7/1
Y1 - 2016/7/1
N2 - During the course of our taxonomic investigation of Helicobacter cinaedi, it was realized that the strains isolated from dogs, which have been identified as H. cinaedi, showed different biochemical traits than did the isolates obtained from humans. None of the three dog isolates could reduce nitrate to nitrite, whereas all of the human H. cinaedi isolates could do so. The dog isolates showed a strong positive alkaline phosphatase reaction and could grow at 42 °C, however the human isolates showed negative to very weak responses to those tests. The GyrA protein based phylogenetic analysis showed that the three isolates from dogs formed a slightly distinct cluster from the human isolate cluster. Phylogenetic analysis of the 16S rRNA, 23S rRNA, gyrB, and hsp60 gene sequences further confirmed that the dog isolates differed from the human H. cinaedi isolate cluster. The whole-genome in silico DNA similarities of each isolate based on their full genome sequences revealed that the isolates from dogs shared more than 94.9% ANIb (average nucleotide identity based on BLAST), while 94.0% ANIb were found between the isolates from dogs and the humans, including the H. cinaedi type strain ATCC BAA-847T (= CCUG 18818T). From these data, we propose a new species, ‘H. canicola’ sp. nov., for the isolates from dogs. The type strain is PAGU 1410T (CCUG 33887T = LMG 29580T).
AB - During the course of our taxonomic investigation of Helicobacter cinaedi, it was realized that the strains isolated from dogs, which have been identified as H. cinaedi, showed different biochemical traits than did the isolates obtained from humans. None of the three dog isolates could reduce nitrate to nitrite, whereas all of the human H. cinaedi isolates could do so. The dog isolates showed a strong positive alkaline phosphatase reaction and could grow at 42 °C, however the human isolates showed negative to very weak responses to those tests. The GyrA protein based phylogenetic analysis showed that the three isolates from dogs formed a slightly distinct cluster from the human isolate cluster. Phylogenetic analysis of the 16S rRNA, 23S rRNA, gyrB, and hsp60 gene sequences further confirmed that the dog isolates differed from the human H. cinaedi isolate cluster. The whole-genome in silico DNA similarities of each isolate based on their full genome sequences revealed that the isolates from dogs shared more than 94.9% ANIb (average nucleotide identity based on BLAST), while 94.0% ANIb were found between the isolates from dogs and the humans, including the H. cinaedi type strain ATCC BAA-847T (= CCUG 18818T). From these data, we propose a new species, ‘H. canicola’ sp. nov., for the isolates from dogs. The type strain is PAGU 1410T (CCUG 33887T = LMG 29580T).
KW - 16S rRNA
KW - 23S rRNA
KW - Average nucleotide identity (ANI)
KW - Canine
KW - DNA gyrase
KW - DNA–DNA hybridization
KW - Heat shock protein
KW - In silico DNA similarity analysis
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U2 - 10.1016/j.syapm.2016.06.004
DO - 10.1016/j.syapm.2016.06.004
M3 - Article
C2 - 27381809
AN - SCOPUS:84991045190
VL - 39
SP - 307
EP - 312
JO - Systematic and Applied Microbiology
JF - Systematic and Applied Microbiology
SN - 0723-2020
IS - 5
ER -