Properties of the electrogenic activity of bacteriorhodopsin

Shizuma Miyazaki, Makoto Matsumoto, Søren Bo Brier, Toshihiro Higaki, Takumi Yamada, Tetsuaki Okamoto, Hiroshi Ueno, Shoichi Toyabe, Eiro Muneyuki

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In this study, we analyzed the photoelectric current generated by bacteriorhodopsin adsorbed on a polymer film, "Lumirror" (Muneyuki et al. in FEBS Lett 427:109-114, 1998). We could examine the photoelectric current over a wide range of light intensity and pH values using the same membrane owing to the mechanical and chemical stability of the thin polymer film. We analyzed the photoelectric current by comparison with a simple equivalent electric circuit. Analysis of experimental results obtained at different light intensities suggested that the electromotive force of the bacteriorhodopsin was independent of light intensity. The pH dependence of the photoelectric current suggested that the bacteriorhodopsin could generate a maximum electromotive force at approximately pH 6.

Original languageEnglish
Pages (from-to)257-265
Number of pages9
JournalEuropean Biophysics Journal
Volume42
Issue number4
DOIs
Publication statusPublished - 2013 Apr
Externally publishedYes

Keywords

  • Electromotive force
  • Equivalent circuit
  • Photoelectric current
  • Proton pump
  • pH dependence

ASJC Scopus subject areas

  • Biophysics

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  • Cite this

    Miyazaki, S., Matsumoto, M., Brier, S. B., Higaki, T., Yamada, T., Okamoto, T., Ueno, H., Toyabe, S., & Muneyuki, E. (2013). Properties of the electrogenic activity of bacteriorhodopsin. European Biophysics Journal, 42(4), 257-265. https://doi.org/10.1007/s00249-012-0870-0