Properties of single chloride channels in primary neuronal cultures of Drosophila

Daisuke Yamamoto; Noboyuki Suzuki

doi:10.1016/0005-2736(89)90289-7

Properties of single chloride channels in primary neuronal cultures of Drosophila

Daisuke Yamamoto, Noboyuki Suzuki

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

An anion channel from Drosophila neurosn had the selectivity sequence: NO₃^-(1.97)>Br^-(1.12) ≈ I^-(1.03) ≈ Cl^-(1)>F^-(0.32) ≫ glutamate(< 0.02) as estimated by the permeability ratio based on the reversal potential measurement. 4-Acetoamido-4′-isothiocyanostilbene-2,2′-disulfonic acid applied internally at 10 μM blocked the channel. We suggest that the chloride channel identified here may provide a pathway for Cl^- in the resting membrane of Drosophila neurons.

Original language	English
Pages (from-to)	187-190
Number of pages	4
Journal	BBA - Biomembranes
Volume	986
Issue number	1
DOIs	https://doi.org/10.1016/0005-2736(89)90289-7
Publication status	Published - 1989 Nov 17

Keywords

(Drosophila)
Channel selectivity
Chloride channel
Membrane potential
SITS
Single-channel conductance

ASJC Scopus subject areas

Biophysics
Biochemistry
Cell Biology

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10.1016/0005-2736(89)90289-7

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abstract = "An anion channel from Drosophila neurosn had the selectivity sequence: NO3-(1.97)>Br-(1.12) ≈ I-(1.03) ≈ Cl-(1)>F-(0.32) ≫ glutamate(< 0.02) as estimated by the permeability ratio based on the reversal potential measurement. 4-Acetoamido-4′-isothiocyanostilbene-2,2′-disulfonic acid applied internally at 10 μM blocked the channel. We suggest that the chloride channel identified here may provide a pathway for Cl- in the resting membrane of Drosophila neurons.",

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T1 - Properties of single chloride channels in primary neuronal cultures of Drosophila

AU - Yamamoto, Daisuke

AU - Suzuki, Noboyuki

PY - 1989/11/17

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AB - An anion channel from Drosophila neurosn had the selectivity sequence: NO3-(1.97)>Br-(1.12) ≈ I-(1.03) ≈ Cl-(1)>F-(0.32) ≫ glutamate(< 0.02) as estimated by the permeability ratio based on the reversal potential measurement. 4-Acetoamido-4′-isothiocyanostilbene-2,2′-disulfonic acid applied internally at 10 μM blocked the channel. We suggest that the chloride channel identified here may provide a pathway for Cl- in the resting membrane of Drosophila neurons.

KW - (Drosophila)

KW - Channel selectivity

KW - Chloride channel

KW - Membrane potential

KW - SITS

KW - Single-channel conductance

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Properties of single chloride channels in primary neuronal cultures of Drosophila

Abstract

Keywords

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