Properties of farnesol phosphokinase of Botryococcus braunii

Hitoshi Inoue; Hiroshi Sagami; Tanetoshi Koyama; Kyozo Ogura

doi:10.1016/0031-9422(95)00285-F

Properties of farnesol phosphokinase of Botryococcus braunii

Hitoshi Inoue, Hiroshi Sagami, Tanetoshi Koyama, Kyozo Ogura

Institute of Multidisciplinary Research for Advanced Materials (IMRAM)

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

A 100 000 g pellet fraction of a strain of Botryococcus braunii B race had the capability of phosphorylating farnesol in the presence of CTP to give its mono- and diphosphate esters, but the same fraction did not phosphorylate other isoprenoid alcohols, including geraniol, geranylgeraniol, Z,E-polyprenols and dolichols. Exogenously added farnesyl monophosphate was not phosphorylated by this fraction. The K_m values in the formation of farnesyl monophosphate were 0.5 μM and 0.2 mM for farnesol and CTP, respectively. Iodoacetamide strongly inhibited the formation of farnesyl diphosphate, but had no effect on that of farnesyl monophosphate.

Original language	English
Pages (from-to)	377-381
Number of pages	5
Journal	Phytochemistry
Volume	40
Issue number	2
DOIs	https://doi.org/10.1016/0031-9422(95)00285-F
Publication status	Published - 1995 Sep

Keywords

Botryococcus braunii
alga
farnesol
farnesyl diphosphate
farnesyl monophosphate
phosphokinase

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Plant Science
Horticulture

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title = "Properties of farnesol phosphokinase of Botryococcus braunii",

abstract = "A 100 000 g pellet fraction of a strain of Botryococcus braunii B race had the capability of phosphorylating farnesol in the presence of CTP to give its mono- and diphosphate esters, but the same fraction did not phosphorylate other isoprenoid alcohols, including geraniol, geranylgeraniol, Z,E-polyprenols and dolichols. Exogenously added farnesyl monophosphate was not phosphorylated by this fraction. The Km values in the formation of farnesyl monophosphate were 0.5 μM and 0.2 mM for farnesol and CTP, respectively. Iodoacetamide strongly inhibited the formation of farnesyl diphosphate, but had no effect on that of farnesyl monophosphate.",

keywords = "Botryococcus braunii, alga, farnesol, farnesyl diphosphate, farnesyl monophosphate, phosphokinase",

author = "Hitoshi Inoue and Hiroshi Sagami and Tanetoshi Koyama and Kyozo Ogura",

year = "1995",

month = sep,

doi = "10.1016/0031-9422(95)00285-F",

language = "English",

volume = "40",

pages = "377--381",

journal = "Phytochemistry",

issn = "0031-9422",

publisher = "Elsevier Limited",

number = "2",

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TY - JOUR

T1 - Properties of farnesol phosphokinase of Botryococcus braunii

AU - Inoue, Hitoshi

AU - Sagami, Hiroshi

AU - Koyama, Tanetoshi

AU - Ogura, Kyozo

PY - 1995/9

Y1 - 1995/9

N2 - A 100 000 g pellet fraction of a strain of Botryococcus braunii B race had the capability of phosphorylating farnesol in the presence of CTP to give its mono- and diphosphate esters, but the same fraction did not phosphorylate other isoprenoid alcohols, including geraniol, geranylgeraniol, Z,E-polyprenols and dolichols. Exogenously added farnesyl monophosphate was not phosphorylated by this fraction. The Km values in the formation of farnesyl monophosphate were 0.5 μM and 0.2 mM for farnesol and CTP, respectively. Iodoacetamide strongly inhibited the formation of farnesyl diphosphate, but had no effect on that of farnesyl monophosphate.

AB - A 100 000 g pellet fraction of a strain of Botryococcus braunii B race had the capability of phosphorylating farnesol in the presence of CTP to give its mono- and diphosphate esters, but the same fraction did not phosphorylate other isoprenoid alcohols, including geraniol, geranylgeraniol, Z,E-polyprenols and dolichols. Exogenously added farnesyl monophosphate was not phosphorylated by this fraction. The Km values in the formation of farnesyl monophosphate were 0.5 μM and 0.2 mM for farnesol and CTP, respectively. Iodoacetamide strongly inhibited the formation of farnesyl diphosphate, but had no effect on that of farnesyl monophosphate.

KW - Botryococcus braunii

KW - alga

KW - farnesol

KW - farnesyl diphosphate

KW - farnesyl monophosphate

KW - phosphokinase

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U2 - 10.1016/0031-9422(95)00285-F

DO - 10.1016/0031-9422(95)00285-F

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AN - SCOPUS:0028887663

VL - 40

SP - 377

EP - 381

JO - Phytochemistry

JF - Phytochemistry

SN - 0031-9422

IS - 2

ER -

Properties of farnesol phosphokinase of Botryococcus braunii

Abstract

Keywords

ASJC Scopus subject areas

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