TY - JOUR
T1 - Promoter trapping in Lotus japonicus reveals novel root and nodule GUS expression domains
AU - Buzas, Diana Mihaela
AU - Lohar, Dasharath
AU - Sato, Shusei
AU - Nakamura, Yasukazu
AU - Tabata, Satoshi
AU - Vickers, Claudia Estelle
AU - Stiller, Jiri
AU - Gresshoff, Peter Michael
N1 - Funding Information:
We gratefully acknowledge Professor Keith Lindsey (University of Durham, UK) for providing the p∆GUSBIN19 binary vector, Dr. Simona Radutoiu and Crina Zaharia for initial transgenic work, Dr. Priya Joshi for the sectioning in Fig. 3A, and Dr. Ulrike Mathesius, Dr. Artem Men, Dr. Mark Kinkema and Dr. Attila Kereszt for help with the manuscript. D.M.B. was supported by an UQIPRS scholarship. This work was supported by the Australian Research Council, National Science Founation (No. MCB-9808625 to P.M.G. and J.S.) and the Australian Research Council (No. DP0210101 to J.S.).
PY - 2005/8
Y1 - 2005/8
N2 - Agrobacterium-based transformation was used to introduce a promoter-less glucuronidase uidA gene (β-glucuronidase; GUS) into Lotus japonicus. Transgenic plants were screened for GUS activation at different stages after inoculation with its symbiont, Mesorhizobium loti. Functional GUS fusion frequencies ranged from about 2 to 5% of the total number of transgenic lines. These lines provide excellent histological markers for tissue ontogeny analysis. Some of the activations generated GUS expression patterns that correspond to well-known tissue types, such as lateral root and nodule primordia, root tips and developing nodules (line CHEETAH). Others generated GUS activation associated with predictable but previously unknown (i) tissue types, such as the vascular bundle of the nodule (line VASCO); or (ii) expression domains, such as pericycle, nodule primordia, nodule and flower connective/ vascular tissue (line FATA MORGANA) or inner root cortex cells in the vicinity of a curled root hair, nodule primordia and nodule cortex (line TIMPA). Putative members of two gene superfamilies, EH (Esp homolog) and AAA ATP-ase (ATPase associated with various cellular activities), were located next to the CHEETAH and VASCO insertions, respectively, and a nodulin gene, LjENOD40-2, was located next to the FATA MORGANA insertion. We utilized promoter GUS fusions to investigate the genetic regulation of LjENOD40-2 and FATA MORGANA GUS. The LjENOD40-2 promoter defined a novel expression domain and the FATA MORGANA nodule expression was reiterated by the 2 kb sequence upstream of the T-DNA insertion.
AB - Agrobacterium-based transformation was used to introduce a promoter-less glucuronidase uidA gene (β-glucuronidase; GUS) into Lotus japonicus. Transgenic plants were screened for GUS activation at different stages after inoculation with its symbiont, Mesorhizobium loti. Functional GUS fusion frequencies ranged from about 2 to 5% of the total number of transgenic lines. These lines provide excellent histological markers for tissue ontogeny analysis. Some of the activations generated GUS expression patterns that correspond to well-known tissue types, such as lateral root and nodule primordia, root tips and developing nodules (line CHEETAH). Others generated GUS activation associated with predictable but previously unknown (i) tissue types, such as the vascular bundle of the nodule (line VASCO); or (ii) expression domains, such as pericycle, nodule primordia, nodule and flower connective/ vascular tissue (line FATA MORGANA) or inner root cortex cells in the vicinity of a curled root hair, nodule primordia and nodule cortex (line TIMPA). Putative members of two gene superfamilies, EH (Esp homolog) and AAA ATP-ase (ATPase associated with various cellular activities), were located next to the CHEETAH and VASCO insertions, respectively, and a nodulin gene, LjENOD40-2, was located next to the FATA MORGANA insertion. We utilized promoter GUS fusions to investigate the genetic regulation of LjENOD40-2 and FATA MORGANA GUS. The LjENOD40-2 promoter defined a novel expression domain and the FATA MORGANA nodule expression was reiterated by the 2 kb sequence upstream of the T-DNA insertion.
KW - ENOD40
KW - GUS expression domain
KW - Lotus japonicus
KW - Nodule development
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U2 - 10.1093/pcp/pci129
DO - 10.1093/pcp/pci129
M3 - Article
C2 - 15899881
AN - SCOPUS:24044521629
VL - 46
SP - 1202
EP - 1212
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 8
ER -