Abstract
Proinsulin C-peptide causes multiple molecular and physiological effects, and improves renal and neuronal dysfunction in patients with diabetes. However, whether C-peptide controls the inhibitor κB (IκB)/NF-κB- dependent transcription of genes, including inflammatory genes is unknown. Here we showed that 1 nM C-peptide increased the expression of cyclooxygenase-2 (COX-2) mRNA and its protein in Swiss 3T3 fibroblasts. Consistently, C-peptide enhanced COX-2 gene promoter-activity, which was inhibited by GF109203X and Go6976, specific PKC inhibitors, and BAY11-7082, a specific nuclear factor-κB (NF-κB) inhibitor, accompanied by increased phosphorylation and degradation of IκB. These results suggest that C-peptide stimulates the transcription of inflammatory genes via activation of a PKC/IκB/NF-κB signaling pathway.
Original language | English |
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Pages (from-to) | 1083-1088 |
Number of pages | 6 |
Journal | Journal of biochemistry |
Volume | 139 |
Issue number | 6 |
DOIs | |
Publication status | Published - 2006 Jun |
Keywords
- C-peptide
- Cyclooxygenase-2
- Diabetes
- NF-κB-dependent transcription
- PKC
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology