TY - JOUR
T1 - Proinflammatory cytokines detectable in synovial fluids from patients with temporomandibular disorders
AU - Takahashi, Tetsu
AU - Kondoh, Toshirou
AU - Fukuda, Masayuki
AU - Yamazaki, Yoshiyuki
AU - Toyosaki, Tomoko
AU - Suzuki, Ryuji
N1 - Funding Information:
The cytokines induced by inflammatory stimulus, known as proinflammatory cytokines, include interleukin (IL)-1, tumor necrosis factor-(TNF) 0¢, IL-6, IL-8, granulocyte-monocyte colony-stimulating factor (GM-CSF), and interferon-(IFN) 7.1-3 These cytokines stimulate the production, release, and/or activation of matrix-degrading enzymes and lead to the production of inflammatory mediators such as prostaglandin and leukotriene. 3,4 Because proinflammatory cytokines have been detected in the synovial fluids of knee joints 5-9 and supernatants of cultured synovial tissue cells, 10,11 these cytokines are likely involved in the pathophysiology of rheumatic arthritis (RA) and osteoarthritis (OA). 3 It has been reported that variable degrees of inflam- Supported in part by Grant-in-Aid for Scientific Research 07838005 from the Ministry of Education, Science and Culture of Japan. aLecturer, Division of Oral and Maxillofacial Surgery, Akita University School of Medicine. bChief, Department of Oral and Maxillofacial Surgery, Yokohama Rosai Hospital. CAssistant Professor, Division of Oral and Maxillofacial Surgery, Akita University School of Medicine. aHead and Associate Professor, Division of Oral and Maxillofacial Surgery, Akita University School of Medicine. eResearch Scientist, Department of Immunologya nd Bioregulation, Shionogi Research Laboratories, Shionogi & Co., Ltd. fChief, Department of Immunology and Bioregulation, Shionogi Research Laboratories, Shionogi & Co., Ltd. Received for publication Jan. 2, 1997; returned for revision June 30, 1997; accepted for publication July 30, 1997. Copyright © 1998 by Mosby, Inc. 1079-2104/98/$5.00 + 0 7/12185189 mation exist in some temporomandibular disorders (TMD). 12,13 In addition, several cytokines including ILl, 14 IL-6,15 and TNF-c¢ 16,17 were shown to exist in the synovial fluid (SF) of the temporomandibular joint (TMJ). These findings suggest that these cytokines may be involved in the pathogenesis of synovitis and resorption of cartilage and bone in the TMJ.
PY - 1998
Y1 - 1998
N2 - Objective. To measure the levels of the proinflammatory cytokines, interleukin (IL)-1β, IL-6, tumor necrosis factor- (TNF)α, IL-8, and interferon- (IFN) γ i synovial fluid samples taken from patients with temporomandibular disorders (TMD). Study design. We studied 6 asymptomatic volunteers and 51 patients with TMD. The IL-1β, IL-6, TNF-α, IL-8, and IFN-γ levels in temporomandibular joint synovial fluid were measured using enzyme-linked immunosorbent assay. Results. Measurable level of at least one cytokine in the synovial fluid was found in 40 (64.5%) of 62 joints in the patients: IL-1β and IFN-γ each detected in 18 (29.0%) of 62 joints; IL-6 in 13 (21.0%) of 62 joints; IL-8 in 11 (19.3%) of 57 joints; and TNF-α in only 5 (8.1%) of 62 joints. None of these cytokines was detectable in the synovial fluid in the control group. Furthermore, there was a strong correlation between the detection of IL-1β and pain in the joint area. Conclusions. These data clearly demonstrate increased levels of several proinflammatory cytokines in certain patients with TMD and suggest that these cytokines may play a role in the pathogenesis of synovitis and degenerative changes of the cartilaginous tissue and bone of the temporomandibular joint.
AB - Objective. To measure the levels of the proinflammatory cytokines, interleukin (IL)-1β, IL-6, tumor necrosis factor- (TNF)α, IL-8, and interferon- (IFN) γ i synovial fluid samples taken from patients with temporomandibular disorders (TMD). Study design. We studied 6 asymptomatic volunteers and 51 patients with TMD. The IL-1β, IL-6, TNF-α, IL-8, and IFN-γ levels in temporomandibular joint synovial fluid were measured using enzyme-linked immunosorbent assay. Results. Measurable level of at least one cytokine in the synovial fluid was found in 40 (64.5%) of 62 joints in the patients: IL-1β and IFN-γ each detected in 18 (29.0%) of 62 joints; IL-6 in 13 (21.0%) of 62 joints; IL-8 in 11 (19.3%) of 57 joints; and TNF-α in only 5 (8.1%) of 62 joints. None of these cytokines was detectable in the synovial fluid in the control group. Furthermore, there was a strong correlation between the detection of IL-1β and pain in the joint area. Conclusions. These data clearly demonstrate increased levels of several proinflammatory cytokines in certain patients with TMD and suggest that these cytokines may play a role in the pathogenesis of synovitis and degenerative changes of the cartilaginous tissue and bone of the temporomandibular joint.
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U2 - 10.1016/S1079-2104(98)90415-2
DO - 10.1016/S1079-2104(98)90415-2
M3 - Article
C2 - 9503445
AN - SCOPUS:0031991632
VL - 85
SP - 135
EP - 141
JO - Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
JF - Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
SN - 2212-4403
IS - 2
ER -