Progress in allergy signal research on mast cells: The role of histamine in goblet cell hyperplasia in allergic airway inflammation - A study using the Hdc knockout mouse

Kohei Yamauchi, Mei Piao Hong, Toshihide Nakadate, Toshiki Shikanai, Yutaka Nakamura, Miyuki Niisato, Harumasa Ito, Takashi Mouri, Hitoshi Kobayashi, Chihaya Maesawa, Takashi Sawai, Hiroshi Otsu, Hiroshi Inoue

Research output: Contribution to journalShort survey

7 Citations (Scopus)

Abstract

Although histamine is a central mediator in the immediate allergic reaction, its role in goblet cell hyperplasia in the airway of asthma is not completely understood. This study was designed to examine the role of histamine in goblet cell hyperplasia using histamine-deficient mice (Hdc-/- mice) with allergic airway inflammation. Wild-type and Hdc-/- C57BL/6 mice were sensitized with ovalbumin (OVA). After two-week exposure to OVA, goblet cell hyperplasia was evaluated. Cell differentials in BALF were analyzed. The mRNAs level of MUC5AC and Gob-5 gene were quantitatively determined. The number of eosinophils in BALF increased in both the wild-type mice and Hdc -/- mice; however, their ratio in Hdc-/- mice was significantly lower than that in the wild-type mice. The mRNA levels of Gob-5 and MUC5AC and the ratio of the goblet cells in the airway epithelium were significantly increased in Hdc-/- mice exposed to OVA compared to the wild-type mice under the same condition. These results suggested that histamine may play a regulatory role in goblet cell hyperplasia in allergic airway inflammation.

Original languageEnglish
Pages (from-to)354-360
Number of pages7
JournalJournal of Pharmacological Sciences
Volume106
Issue number3
DOIs
Publication statusPublished - 2008 Apr 8

Keywords

  • Airway remodeling
  • Allergy
  • Asthma
  • Gob-5
  • MUC5AC

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

Fingerprint Dive into the research topics of 'Progress in allergy signal research on mast cells: The role of histamine in goblet cell hyperplasia in allergic airway inflammation - A study using the Hdc knockout mouse'. Together they form a unique fingerprint.

  • Cite this

    Yamauchi, K., Hong, M. P., Nakadate, T., Shikanai, T., Nakamura, Y., Niisato, M., Ito, H., Mouri, T., Kobayashi, H., Maesawa, C., Sawai, T., Otsu, H., & Inoue, H. (2008). Progress in allergy signal research on mast cells: The role of histamine in goblet cell hyperplasia in allergic airway inflammation - A study using the Hdc knockout mouse. Journal of Pharmacological Sciences, 106(3), 354-360. https://doi.org/10.1254/jphs.FM0070262