Progenitor cells from dental follicle are able to form cementum matrix in vivo

Keisuke Handa, Masahiro Saito, Akira Tsunoda, Masato Yamauchi, Shintaro Hattori, Sadao Sato, Minoru Toyoda, Toshio Teranaka, A. Sampath Narayanan

Research output: Contribution to journalArticlepeer-review

95 Citations (Scopus)

Abstract

To address the molecular mechanisms of cementogenesis, we have isolated dental follicle cells and examined them to see if they contain cementoblast progenitors. Dental follicle tissue was dissected from the root surface of bovine tooth germ and cells were released by digestion with bacterial collagenase. The released cells were maintained as a bovine dental follicle cells (BDFC). To elucidate the differentiation capacity of BDFC, they were transplanted into severe combined immunodeficiency (SCID) mice for 4 weeks. Transplanted BDFC formed cementumlike matrix; in contrast, bovine alveolar osteoblast (BAOB) transplants formed bonelike matrix, and bovine periodontal ligament cells (BPDL) formed a small amount of the cementumlike matrix. Immunohistochemical analysis showed that cementumlike matrix was positive for anticementum attachment protein monoclonal antibody, whereas bonelike matrix was negative. These results indicated that the BDFC contained cementoblast progenitors that were able to differentiate to cementoblasts in vivo. They also indicated that the BDFC are phenotypically distinct from BAOB and BPDL, and provide a useful model for investigating molecular mechanisms of cementogenesis.

Original languageEnglish
Pages (from-to)406-408
Number of pages3
JournalConnective Tissue Research
Volume43
Issue number2-3
Publication statusPublished - 2002 Jul 30
Externally publishedYes

Keywords

  • Cementoblasts
  • Cementum
  • Differentiation
  • In vivo transplantation
  • Tooth germ

ASJC Scopus subject areas

  • Rheumatology
  • Biochemistry
  • Orthopedics and Sports Medicine
  • Molecular Biology
  • Cell Biology

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