Profiles of cytokines produced by CD4-positive T lymphocytes stimulated by anti-CD3 antibody in patients with chronic hepatitis C

Koju Kobayashi, Motoyasu Ishii, Takehiko Igarashi, Toshihiro Satoh, Yutaka Miyazaki, Yoshiaki Yajima, Katsuaki Ukai, Hiroshi Suzuki, Atushi Kanno, Yoshiyuki Ueno, Toshihiko Miura, Takayoshi Toyota

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

Helper T cells (Th) are classified as type 1 (Th1) and type 2 (Th2) according to the cytokines they produce; interferon-γ is produced by Th1, and interleukin-4 by Th2. We counted the circulating CD4-positive Th cells that produce interferon-γ or interleukin-4 with an enzyme-linked immunospot assay. CD4-positive T cells isolated from patients with chronic hepatitis B (n = 10), chronic hepatitis C (n = 16), and healthy subjects (n = 10) were stimulated with anti-CD3 antibody in vitro. The number of interferon-γ-producing Th cells was significantly lower in patients with chronic hepatitis C than in healthy subjects (P = 0.0024), whereas in patients with chronic hepatitis B, the number was similar to that in healthy subjects (P = 0.8530). The number of interleukin-4-producing Th cells was significantly higher in patients with chronic hepatitis C (P = 0.0010) and chronic hepatitis B (P = 0.0089) than in healthy subjects. In chronic hepatitis C, the number of interferon-γ-producing Th cells was increased after incubation of the cells with interferon-α (P = 0.008) or with recombinant interferon-γla (P = 0.024), but not with interferon-β (P = 0.051). The number of interleukin-4-producing Th cells was decreased after incubation with interferon-α (P = 0.0004), with interferon-β (P = 0.003), and with recombinant interferon-γla (P = 0.0004). Changes in the numbers of interferon-γ- or interleukin-4-producing Th cells in vitro were more evident in sustained responders to interferon therapy than in non-responders. These results suggest that Th2 cells are the predominant cell type in chronic hepatitis C, and that their activity may be suppressed by the administration of interferon.

Original languageEnglish
Pages (from-to)500-507
Number of pages8
JournalJournal of gastroenterology
Volume33
Issue number4
DOIs
Publication statusPublished - 1998 Aug

Keywords

  • Chronic hepatitis C
  • IL-4-producing Th cell
  • INF-γ-producing Th cells

ASJC Scopus subject areas

  • Gastroenterology

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