Production of truncated protein by the crosslink formation of mRNA with 2′-OMe oligoribonucleotide containing 2-amino-6-vinylpurine

Shinya Hagihara, Shuhei Kusano, Wei Chen Lin, Xiao Guang Chao, Tsuneaki Hori, Shuhei Imoto, Fumi Nagatsugi

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

The development of convenient methods for controlling the protein expression is an important challenge in the postgenomic era. We applied the crosslink forming oligonucleotide (CFO) as a terminator of the ribosomal translation. In this study, we demonstrated that the improved reactivity of our CFO under physiological conditions enabled the sequence-specific introduction of a steric block for a ribosome on mRNAs. In vitro and in cell translation experiments revealed that the crosslinked mRNA can produce the truncated proteins in which the translation terminates at the desired position.

Original languageEnglish
Pages (from-to)3870-3872
Number of pages3
JournalBioorganic and Medicinal Chemistry Letters
Volume22
Issue number12
DOIs
Publication statusPublished - 2012 Jun 15

Keywords

  • Antisense
  • Crosslink
  • Translational inhibition
  • Truncated proteins

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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