Production of somatic hybrid plants, 'Brassicomoricandia', through protoplast fusion between Moricandia arvensis and Brassica oleracea

Kinya Toriyama, Kokichi Hinata, Toshiaki Kameya

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Intergeneric hybrid plants were obtained through protoplast fusion between Brassica oleracea L. and Moricandia arvensis (L.) DC., members of the Brassicaceae. Protoplast fusion was carried out by the dextran method using protoplasts isolated from hypocotyls of red cabbage and from suspended cells of M. arvensis. The fusion-treated protoplasts cultured in B5 medium with high concentrations of 2,4-dichlorophenoxyacetic acid and 6-benzylaminopurine produced abundant calli, and out of them, 8 calli produced a number of hybrid plants. Some plants have already come into flower. All the regenerated plants exhibited combined morphological characteristics of both parental species. Analysis of leaf isozymes for acid phosphatase and aspartate aminotransferase also indicated their hybridity. Even though no selection was performed for hybrid cells, all the regenerated plants were hybrids. It is thought that the somatic hybrids exhibited hybrid vigour and readily produced shoots. Two somatic hybrid plants were also obtained between cauliflower and M. arvensis.

Original languageEnglish
Pages (from-to)123-128
Number of pages6
JournalPlant Science
Volume48
Issue number2
DOIs
Publication statusPublished - 1987 Jan 1

Keywords

  • Brassica oleracea
  • Moricandia arvensis
  • protoplast fusion
  • somatic hybrid
  • tissue culture

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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