Production of small nano-sized particles by complex formation between polycations and linearized plasmid DNA at a low pH

Ryohei Togashi; Hidetaka Akita; Hideyoshi Harashima

doi:10.1016/j.jbiosc.2013.04.009

Production of small nano-sized particles by complex formation between polycations and linearized plasmid DNA at a low pH

Ryohei Togashi, Hidetaka Akita, Hideyoshi Harashima

Research output: Contribution to journal › Article › peer-review

Abstract

We report on the technical advance of linearized pDNA (pDNA_linear) above the circular one (pDNA_circ) for preparation of small-sized DNA/polycation complexes (DPC) at a low pH. Also, the resistance of the DPC formed with pDNA_linear against poly-l-asparagine indicates that effective ion-pairing occurred between the pDNA_linear and polycations.

Original language	English
Pages (from-to)	528-531
Number of pages	4
Journal	Journal of Bioscience and Bioengineering
Volume	116
Issue number	4
DOIs	https://doi.org/10.1016/j.jbiosc.2013.04.009
Publication status	Published - 2013 Oct
Externally published	Yes

Keywords

Compaction
Linearized DNA
PH
Plasmid DNA
Polycation

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1016/j.jbiosc.2013.04.009

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title = "Production of small nano-sized particles by complex formation between polycations and linearized plasmid DNA at a low pH",

abstract = "We report on the technical advance of linearized pDNA (pDNAlinear) above the circular one (pDNAcirc) for preparation of small-sized DNA/polycation complexes (DPC) at a low pH. Also, the resistance of the DPC formed with pDNAlinear against poly-l-asparagine indicates that effective ion-pairing occurred between the pDNAlinear and polycations.",

keywords = "Compaction, Linearized DNA, PH, Plasmid DNA, Polycation",

author = "Ryohei Togashi and Hidetaka Akita and Hideyoshi Harashima",

note = "Funding Information: This work is supported by Funding Program for Next Generation World-Leading Researchers (NEXT Program) . H.A. is also supported by the Asahi Glass Foundation . The authors would also like to thank Dr. M. S. Feather for his helpful advice in writing the English manuscript.",

year = "2013",

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doi = "10.1016/j.jbiosc.2013.04.009",

language = "English",

volume = "116",

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journal = "Journal of Bioscience and Bioengineering",

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T1 - Production of small nano-sized particles by complex formation between polycations and linearized plasmid DNA at a low pH

AU - Togashi, Ryohei

AU - Akita, Hidetaka

AU - Harashima, Hideyoshi

N1 - Funding Information: This work is supported by Funding Program for Next Generation World-Leading Researchers (NEXT Program) . H.A. is also supported by the Asahi Glass Foundation . The authors would also like to thank Dr. M. S. Feather for his helpful advice in writing the English manuscript.

PY - 2013/10

Y1 - 2013/10

N2 - We report on the technical advance of linearized pDNA (pDNAlinear) above the circular one (pDNAcirc) for preparation of small-sized DNA/polycation complexes (DPC) at a low pH. Also, the resistance of the DPC formed with pDNAlinear against poly-l-asparagine indicates that effective ion-pairing occurred between the pDNAlinear and polycations.

AB - We report on the technical advance of linearized pDNA (pDNAlinear) above the circular one (pDNAcirc) for preparation of small-sized DNA/polycation complexes (DPC) at a low pH. Also, the resistance of the DPC formed with pDNAlinear against poly-l-asparagine indicates that effective ion-pairing occurred between the pDNAlinear and polycations.

KW - Compaction

KW - Linearized DNA

KW - PH

KW - Plasmid DNA

KW - Polycation

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JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

IS - 4

ER -

Production of small nano-sized particles by complex formation between polycations and linearized plasmid DNA at a low pH

Abstract

Keywords

ASJC Scopus subject areas

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