Production of mouse adiponectin, an anti-diabetic protein, in transgenic sweet potato plants

Thomas Berberich, Toshiyuki Takagi, Atsushi Miyazaki, Motoyasu Otani, Takiko Shimada, Tomonobu Kusano

    Research output: Contribution to journalArticle

    17 Citations (Scopus)

    Abstract

    Adiponectin is a 30 kDa protein exclusively produced and secreted from adipocytes and as a cytokine has been found to link obesity, insulin resistance, and type 2 diabetes. Production of biologically active adiponectin in large scale is desirable for pharmaceutical applications. Mouse adiponectin cDNA was used for developing transgenic sweet potato plants via Agrobacterium-mediated transformation. The presence of the transgene was verified by PCR and DNA gel blot analysis. Further investigated were five independent transgenic lines, all of which expressed high levels of adiponectin mRNA. Immuno blot analysis with a mouse adiponectin antiserum revealed that, in addition to a 29 kDa-protein which co-migrates with the adiponectin protein produced in Escherichia coli cells, a 31 kDa-protein was produced, indicative of a post-translational modification of the protein. The transgenic plants did not show obvious differences in growth rate and morphology in response to adiponectin production.

    Original languageEnglish
    Pages (from-to)1169-1176
    Number of pages8
    JournalJournal of Plant Physiology
    Volume162
    Issue number10
    DOIs
    Publication statusPublished - 2005 Oct 14

    Keywords

    • Adiponectin
    • Agrobacterium-mediated transformation
    • Diabetes
    • Ipomoea batatas
    • Transgenic plant

    ASJC Scopus subject areas

    • Physiology
    • Agronomy and Crop Science
    • Plant Science

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  • Cite this

    Berberich, T., Takagi, T., Miyazaki, A., Otani, M., Shimada, T., & Kusano, T. (2005). Production of mouse adiponectin, an anti-diabetic protein, in transgenic sweet potato plants. Journal of Plant Physiology, 162(10), 1169-1176. https://doi.org/10.1016/j.jplph.2005.01.009