TY - JOUR
T1 - Production of monoclonal antibodies reactive with a denatured form of the friend murine leukemia virus gp70 envelope protein
T2 - Use in a focal infectivity assay, immunohistochemical studies, electron microscopy and western blotting
AU - Robertson, Michael N.
AU - Miyazawa, Masaaki
AU - Mori, Shiro
AU - Caughey, Byron
AU - Evans, Leonard H.
AU - Hayes, Stanley F.
AU - Chesebro, Bruce
PY - 1991/10
Y1 - 1991/10
N2 - Four monoclonal antibodies were selected for their ability to recognize the envelope protein of Friend murine leukemia virus (F-MuLV) in methanol-fixed tissue culture cells. Each of these monoclonal antibodies was found to react only with F-MuLV. By using recombinant retroviruses, it was determined that each of the monoclonal antibodies recognized the C-terminal one-third of the F-MuLV gp70 envelope protein. The monoclonal antibodies were effective in radioimmunoprecipitation of F-MuLV proteins, and one of the antibodies, 720, was also effective in Western blotting. The ability of antibody 720 to react with F-MuLV in methanol-fixed cells facilitated the use of a sensitive immunoperoxidase method with a focal virus infectivity assay. In immunohistochemical studies using light microscopy, antibody 720 could specifically label F-MuLV-infected cells in acetone-fixed tissue sections from F-MuLV-infected animals. Finally, in immuno-gold labelling studies using electron microscopy, antibody 720 could be used to distinguish F-MuLV from amphotropic MuLV.
AB - Four monoclonal antibodies were selected for their ability to recognize the envelope protein of Friend murine leukemia virus (F-MuLV) in methanol-fixed tissue culture cells. Each of these monoclonal antibodies was found to react only with F-MuLV. By using recombinant retroviruses, it was determined that each of the monoclonal antibodies recognized the C-terminal one-third of the F-MuLV gp70 envelope protein. The monoclonal antibodies were effective in radioimmunoprecipitation of F-MuLV proteins, and one of the antibodies, 720, was also effective in Western blotting. The ability of antibody 720 to react with F-MuLV in methanol-fixed cells facilitated the use of a sensitive immunoperoxidase method with a focal virus infectivity assay. In immunohistochemical studies using light microscopy, antibody 720 could specifically label F-MuLV-infected cells in acetone-fixed tissue sections from F-MuLV-infected animals. Finally, in immuno-gold labelling studies using electron microscopy, antibody 720 could be used to distinguish F-MuLV from amphotropic MuLV.
KW - Electron microscopy
KW - Immunohistochemistry
KW - Monoclonal antibody
KW - Retrovirus
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U2 - 10.1016/0166-0934(91)90105-9
DO - 10.1016/0166-0934(91)90105-9
M3 - Article
C2 - 1744218
AN - SCOPUS:0025953541
VL - 34
SP - 255
EP - 271
JO - Journal of Virological Methods
JF - Journal of Virological Methods
SN - 0166-0934
IS - 3
ER -