Production of l-theanine using glutaminase encapsulated in carbon-coated mesoporous silica with high pH stability

Tetsuji Itoh, Yasuto Hoshikawa, Shun Ichi Matsuura, Junko Mizuguchi, Hiroyuki Arafune, Taka Aki Hanaoka, Fujio Mizukami, Akari Hayashi, Hirotomo Nishihara, Takashi Kyotani

    Research output: Contribution to journalArticlepeer-review

    26 Citations (Scopus)

    Abstract

    A new enzymatic process for production of l-theanine was presented using glutaminase combined with immobilization technique on carbon-coated mesoporous silica (carbon/SBA-15). For the attempt to enhance the durability against high pH conditions, the pore surface of mesoporous silica SBA-15 was coated with a thin layer of carbon. Carbon coating was done by addition of 2,3-dihydroxynaphthalene (DN) on the pore surface, followed by a dehydration reaction between surface silanol groups in SBA-15 and hydroxyl groups of DN molecules, and further carbonization of DN. A glutaminase was confined in nanospace (about 5. nm) of carbon/SBA-15. The resulting product, glutaminase/carbon/SBA-15, successfully catalyzed the reaction for production of l-theanine under high pH conditions.

    Original languageEnglish
    Pages (from-to)207-214
    Number of pages8
    JournalBiochemical Engineering Journal
    Volume68
    DOIs
    Publication statusPublished - 2012 Oct 15

    Keywords

    • Carbon
    • Enzyme immobilization
    • Mesoporous silica

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Environmental Engineering
    • Biomedical Engineering

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