Production of endothelin by vascular smooth muscle cells

S. M. Kanse, K. Takahashi, J. B. Warren, T. Perera, M. Porta, M. Ghatei, S. R. Bloom

Research output: Contribution to journalArticlepeer-review

41 Citations (Scopus)

Abstract

Endothelin (ET) production by cultured vascular smooth muscle cells isolated from rat and rabbit aortae was measured by a specific radioimmunoassay. Vascular smooth muscle cells released ET at a rate of 0.6 (range of 0.1-1) fmol/105cells/24 h compared to 45 (range of 10-80) fmol/105cells/h for endothelial cells. ET immunoreactivity was confirmed as ET-1 by fast protein liquid chromatography. Calcitonin generelated peptide (CGRP) and vasoactive intestinal polypeptide (VIP) suppressed basal release of ET by 24-66% from both rat and rabbit vascular smooth muscle. Forskolin and dibutryl-cAMP similarly suppressed ET release by 33-86%, suggesting that increased intracellular cAMP may account for the mechanism of action of CGRP and VIP. In contrast, CGRP and VIP did not have any effect on ET release from endothelial cells. The characteristics and regulation of ET binding sites on vascular smooth muscle cells were measured using [125I]ET-1 as the radioligand. Pretreatment with CGRP, VIP, forskolin, and dibutryl-cAMP increased the ET receptor density on both smooth muscle cell types. The low level production of ET-1 by vascular smooth muscle cells may have an important autocrine function and may be under regulation of neuropeptides localized to perivascular nerves.

Original languageEnglish
Pages (from-to)S113-S116
JournalJournal of cardiovascular pharmacology
Volume17
DOIs
Publication statusPublished - 1991

Keywords

  • Autocrine
  • Endothelin
  • Receptors
  • Vascular smooth muscle

ASJC Scopus subject areas

  • Pharmacology
  • Cardiology and Cardiovascular Medicine

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