Production of biodiesel from plant oil hydrolysates using an Aspergillus oryzae whole-cell biocatalyst highly expressing Candida antarctica lipase B

Daisuke Adachi, Shinji Hama, Kazunori Nakashima, Takayuki Bogaki, Chiaki Ogino, Akihiko Kondo

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

For enzymatic biodiesel production from plant oil hydrolysates, an Aspergillus oryzae whole-cell biocatalyst that expresses Candida antarctica lipase B (r-CALB) with high esterification activity was developed. Each of soybean and palm oils was hydrolyzed using Candida rugosa lipase, and the resultant hydrolysates were subjected to esterification where immobilized r-CALB was used as a catalyst. In esterification, r-CALB afforded a methyl ester content of more than 90% after 6. h with the addition of 1.5. M equivalents of methanol. Favorably, stepwise additions of methanol and a little water were unnecessary for maintaining the lipase stability of r-CALB during esterification. During long-term esterification in a rotator, r-CALB can be recycled for 20 cycles without a significant loss of lipase activity, resulting in a methyl ester content of more than 90% even after the 20th batch. Therefore, the presented reaction system using r-CALB shows promise for biodiesel production from plant oil hydrolysates.

Original languageEnglish
Pages (from-to)410-416
Number of pages7
JournalBioresource Technology
Volume135
DOIs
Publication statusPublished - 2013 May

Keywords

  • Biodiesel fuel
  • Biomass support particles
  • Esterification
  • Immobilized cells
  • Lipase

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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