Production of bioactive bovine fibroblast growth factor 4 in E.coli based on the common nucleotide sequence of its structural gene in three breeds

Saiko Sugawara, Toshihiko Ito, Sho Sato, Mari Yokoo, Yuki Mori, Kano Kasuga, Ikuo Kojima, Tomokazu Fukuda, Ken ichi Yamanaka, Miki Sakatani, Masashi Takahashi, Masayuki Kobayashi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Fibroblast growth factor 4 (FGF4) is considered a crucial gene in the proper development of bovine embryos. We recently determined the FGF4 gene sequence in eight cattle derived from three breeds and revealed a common nucleotide sequence of the structural gene encoding FGF4, which leads to the deletion and mutation of amino acid sequences in the mature FGF4 (Pro32-Leu206) compared with the sequence previously reported. In the present study, HisbFGF4, a 6× histidine-tagged bovine FGF4 (Pro32-Leu206), was produced in Escherichiacoli based on the validated nucleotide sequence and purified by heparin column chromatography. In primary bovine fibroblasts, HisbFGF4 showed significant mitogenic activity, whereas, intriguingly, the activity of a commercially available recombinant human FGF4 (Gly25-Leu206) produced in E. coli was weaker than that of HisbFGF4. In conclusion, the present study provides a simple method for the production of a bioactive bovine FGF4 derivative in E. coli utilizing its structural gene elucidated by us.

Original languageEnglish
Pages (from-to)275-280
Number of pages6
JournalAnimal Science Journal
Volume84
Issue number3
DOIs
Publication statusPublished - 2013 Mar 1

Keywords

  • Bovine
  • E.coli
  • Fibroblast growth factor 4
  • Recombinant

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

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    Sugawara, S., Ito, T., Sato, S., Yokoo, M., Mori, Y., Kasuga, K., Kojima, I., Fukuda, T., Yamanaka, K. I., Sakatani, M., Takahashi, M., & Kobayashi, M. (2013). Production of bioactive bovine fibroblast growth factor 4 in E.coli based on the common nucleotide sequence of its structural gene in three breeds. Animal Science Journal, 84(3), 275-280. https://doi.org/10.1111/asj.12036