Production of anti-amyloid /? Antibodies in mice fed rice expressing amyloid /?

Jun Nojima, Rika Ishii-Katsuno, Eugene Futai, Noboru Sasagawa, Yuichiro Watanabe, Taiji Yoshida, Shoichi Ishiura

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The main signs of Alzheimer's disease (AD) are cognitive impairment and senile plaques composed of amyloid beta (A/3) observed in patients' brains. Therefore, therapy for AD focuses on the removal of A/?. We developed an "edible vaccine" that employs intestinal immunity with little to no side effects. Rice was utilized as an edible vaccine. It expressed GFP-A/342. A/} rice was administered orally to wild-type (WT) mice causing production of anti-A/3 antibodies. Since A/} rice was mixed with the cholera toxin B subunit (CTB), antibody against the rice seed protein was also produced. Then, mice were caused to develop immune tolerance against the rice seed protein by oral administration of A/? rice mixed with CTB. The results indicated that only anti-A/? antibodies were produced.

Original languageEnglish
Pages (from-to)396-400
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Volume75
Issue number2
DOIs
Publication statusPublished - 2011 Mar 7
Externally publishedYes

Keywords

  • Alzheimer's disease
  • Amyloid beta
  • An edible vaccine
  • Immune toleranc

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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  • Cite this

    Nojima, J., Ishii-Katsuno, R., Futai, E., Sasagawa, N., Watanabe, Y., Yoshida, T., & Ishiura, S. (2011). Production of anti-amyloid /? Antibodies in mice fed rice expressing amyloid /? Bioscience, Biotechnology and Biochemistry, 75(2), 396-400. https://doi.org/10.1271/bbb.100861