Production of 8-nitro-cGMP in osteocytic cells and its upregulation by parathyroid hormone and prostaglandin E 2

Kazuhiro Nagayama, Yoichi Miyamoto, Kotaro Kaneko, Kentaro Yoshimura, Kiyohito Sasa, Takaaki Akaike, Shigemoto Fujii, Eri Izumida, Risa Uyama, Daichi Chikazu, Koutaro Maki, Ryutaro Kamijo

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


Osteocytes regulate bone remodeling, especially in response to mechanical loading and unloading of bone, with nitric oxide reported to play an important role in that process. In the present study, we found that 8-nitroguanosine 3′,5′-cyclic monophosphate (8-nitro-cGMP), a second messenger of nitric oxide in various types of cells, was produced by osteocytes in bone tissue as well as cultured osteocytic Ocy454 cells. The amount of 8-nitro-cGMP in Ocy454 cells increased during incubation with parathyroid hormone or prostaglandin E 2 , both of which are known to upregulate receptor activator of nuclear factor-κB ligand (RANKL) mRNA expression in osteocytes. On the other hand, exogenous 8-nitro-cGMP did not have effects on either the presence or absence of these bioactive substances. Furthermore, neither an inhibitor of nitric oxide synthase nor 8-bromo-cGMP, a cell-permeable analog of cGMP, showed remarkable effects on mRNA expression of sclerostin or RANKL. These results indicate that neither nitric oxide nor its downstream compounds, including 8-nitro-cGMP, alone are sufficient for induction of functional changes in osteocytes.

Original languageEnglish
Pages (from-to)45-51
Number of pages7
JournalIn Vitro Cellular and Developmental Biology - Animal
Issue number1
Publication statusPublished - 2019 Jan 15


  • 8-Nitro-cGMP
  • Nitric oxide
  • Osteocytes
  • Parathyroid hormone

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology


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