Production and product quality assessment of human hepatitis B virus pre-S2 antigen in submerged and solid-state cultures of Aspergillus oryzae

Jun Ichi Maruyama, Hitoshi Ohnuma, Akira Yoshikawa, Hiroshi Kadokura, Harushi Nakajima, Katsuhiko Kitamoto

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Pre-S2 is a diagnostically important antigen of human hepatitis B virus (HBV). In order to produce pre-S2 antigen in Aspergillus oryzae, the gene [pre-S2]3, which encodes a tandemly triplicated repeat of pre-S2 polypeptides was fused with the partial glaA gene encoding glucoamylase lacking the starch-binding domain. In submerged culture, A. oryzae transformants carrying glaA-[pre-S2]3 secreted a heterogeneously glycosylated form of the fusion protein that was partially degraded. Contrarily, utilization of a wheat bran solid-state culture system resulted in the secretion of a homogeneous glycosylated form of the whole fusion protein. This is the first report of a dissimilarity in glycosylated modification between submerged and solid-state culture conditions in heterologous protein production in A. oryzae.

Original languageEnglish
Pages (from-to)118-120
Number of pages3
JournalJournal of Bioscience and Bioengineering
Volume90
Issue number1
DOIs
Publication statusPublished - 2000 Jan 1
Externally publishedYes

Keywords

  • Aspergillus oryzae
  • Human hepatitis B virus
  • Pre-S2
  • Solid-state culture
  • Submerged culture

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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