Production and characterization of new monoclonal antibodies that distinguish subsets of mink lymphoid cells

M. Miyazawa, S. Mori, G. J. Spangrude, J. B. Wolfinbarger, M. E. Bloom

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Several hybridoma clones that produce monoclonal antibodies (MAbs) reacting with subpopulations of mink lymphoid cells were established. Two of the MAbs, MTS-4.3 and MTS-9.3, reacted with relatively small populations of surface immunoglobulin (Ig)-negative (Ig-) lymphocytes. MTS-4.3+ and MTS- 9.3+ cells were distributed in the thymic cortex and medulla, paracortical areas of lymph nodes, and periarterial lymphoid sheaths of the spleen, indicating that these MAbs identify T lymphocytes. Another MAb, MTB-5.6, reacted with a large proportion of surface Ig+ lymph node cells, but not with surface Ig- cells. In immunohistochemistry this MAb stained dendritic epithelial cells of thymic cortex, large polygonal cells of thymic medulla, a large proportion of lymphocytes in the mantle zone of lymphoid follicles, dendritic-shaped cells of paracortical area, and some lymphocytes and macrophage-like cells of medullary cords and sinuses of lymph nodes. The expression of the cell-surface antigen reacting with MTB-5.6 on Ig+ lymph node cells was increased after concanavalin A stimulation. These new reagents may be useful to analyze cellular basis of the abnormal immune responses observed in Aleutian mink disease, a classical model of human autoimmune diseases.

Original languageEnglish
Pages (from-to)107-114
Number of pages8
JournalHybridoma
Volume13
Issue number2
DOIs
Publication statusPublished - 1994

ASJC Scopus subject areas

  • Immunology
  • Genetics

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