Process of Bonelike Apatite Formation on Sintered Hydroxyapatite in Serum-Containing SBF

Tadashi Kokubo, Teruyuki Himeno, Hyun Min Kim, Masakazu Kawashita, Takashi Nakamura

Research output: Contribution to journalConference articlepeer-review

5 Citations (Scopus)


It has been revealed that even sintered hydroxyapatite forms a bonelike apatite layer on its surface in the living body and bonds to surround bone through the apatite layer. In the previous study, process on bonelike apatite formation on sintered hydroxyapatite was investigated in a protein-free acellular simulated body fluid (SBF) with ion concentrations nearly equal to those of the human blood plasma. In the present study, the effect of addition of serum to SBF on the process of bonelike apatite formation was investigated by using transmission electron microscopy attached with energy dispersive X-ray spectroscopy (TEM-EDX) and laser electrophoresis spectroscopy. In this solution similar to in the original SBF, the sintered hydroxyapatite formed the bonelike apatite on its surface via formation of Ca-rich hydroxyapatite and amorphous calcium phosphate with low Ca/P ratio. The rate of these structural changes in the serum-containing SBF was, however, lower than that in the serum-free SBF. This was attributed to lower negative charging of the surface of the sintered hydroxyapatite at the initial stage due to adsorption of positively charged globulin and lower Ca2+ ion concentrations in SBF due to its combination with negatively charged albumin; in serum-containing SBF.

Original languageEnglish
Pages (from-to)139-142
Number of pages4
JournalKey Engineering Materials
Publication statusPublished - 2004 Jan 1
EventThe Annual Meeting of the International Society for Ceramics in Medicine - Porto, Portugal
Duration: 2003 Nov 62003 Nov 9


  • Bonelike apatite
  • EDX
  • SBF
  • Serum
  • Sintered hydroxyapatite
  • TEM
  • Zeta-potential

ASJC Scopus subject areas

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering


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