Probing of the secondary structure of maxizymes.

J. M. Zhou, Y. Nakamatsu, T. Kuwabara, M. Warashina, Y. Tanaka, K. Yoshinari, K. Taira

Research output: Contribution to journalArticlepeer-review

Abstract

The protein encoded by chimeric BCR-ABL mRNA causes chronic myelogenous leukemia (CML). We showed previously that a novel allosterically controllable ribozyme, of the type known as a maxizyme, can cleave this mRNA, with high specificity and high-level activity in vivo. In order to probe the putative conformational changes, we used a weakly alkaline solution to hydrolyze differentially phosphodiester bonds that were located in different environments. As indicated by earlier data obtained in vivo, our results demonstrated that the active conformation was achieved only in the presence of the junction within the chimeric BCR-ABL mRNA.

Original languageEnglish
Pages (from-to)219-220
Number of pages2
JournalNucleic acids symposium series
Issue number42
DOIs
Publication statusPublished - 1999

ASJC Scopus subject areas

  • Medicine(all)

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