TY - JOUR
T1 - PRL-1, a protein tyrosine phosphatase, is expressed in neurons and oligodendrocytes in the brain and induced in the cerebral cortex following transient forebrain ischemia
AU - Takano, Seiichirou
AU - Fukuyama, Hidenao
AU - Fukumoto, Manabu
AU - Kimura, Jun
AU - Xue, Jing Hui
AU - Ohashi, Hiromi
AU - Fujita, Jun
N1 - Funding Information:
We thank Dr. T. Harada, Kyoto University, for kind supply of total RNA extracted from regenerating liver. This work was supported in part by Grants-in-Aid from the Ministry of Education, Science and Culture of Japan and a grant from the Chiyoda Mutual Life Foundation.
PY - 1996/8
Y1 - 1996/8
N2 - Protein tyrosine phosphorylation is thought to play an important role in the regulation of neural function. We reported previously that CL100, a cytoplasmic type protein tyrosine phosphatase (PTP), was induced after transient forebrain ischemia. In the present study, changes in the mRNA levels after ischemia of PRL-1, a cytoplasmic type PTP and immediate-early gene similar to CL100, was examined. In situ hybridization histochemistry showed that PRL-1 mRNA was expressed in normal adult rats in neurons and oligodendrocytes in widespread regions including the cerebral cortex, hippocampus and cerebellum. PRL-1 mRNA was expressed in the developing brains on embryonic days 15 and 19 and postnatal day 1. Northern blot analysis showed that PRL-1 mRNA was induced from 6 h to 9 h after reperfusion in the cerebral cortex of postischemic rats. These findings suggest that PRL-1 plays a role in neurons and oliogodendrocytes, and that expression of PRL-1 mRNA is regulated by a mechanism different from those of other immediate-early genes such as c-fos and c-jun.
AB - Protein tyrosine phosphorylation is thought to play an important role in the regulation of neural function. We reported previously that CL100, a cytoplasmic type protein tyrosine phosphatase (PTP), was induced after transient forebrain ischemia. In the present study, changes in the mRNA levels after ischemia of PRL-1, a cytoplasmic type PTP and immediate-early gene similar to CL100, was examined. In situ hybridization histochemistry showed that PRL-1 mRNA was expressed in normal adult rats in neurons and oligodendrocytes in widespread regions including the cerebral cortex, hippocampus and cerebellum. PRL-1 mRNA was expressed in the developing brains on embryonic days 15 and 19 and postnatal day 1. Northern blot analysis showed that PRL-1 mRNA was induced from 6 h to 9 h after reperfusion in the cerebral cortex of postischemic rats. These findings suggest that PRL-1 plays a role in neurons and oliogodendrocytes, and that expression of PRL-1 mRNA is regulated by a mechanism different from those of other immediate-early genes such as c-fos and c-jun.
KW - Cerebral cortex
KW - Neuron
KW - Oligodendrocyte
KW - PRL-1
KW - Protein tyrosine phosphatase
KW - Transient forebrain ischemia
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U2 - 10.1016/0169-328X(96)00035-6
DO - 10.1016/0169-328X(96)00035-6
M3 - Article
C2 - 8840018
AN - SCOPUS:0030221650
VL - 40
SP - 105
EP - 115
JO - Molecular Brain Research
JF - Molecular Brain Research
SN - 0006-8993
IS - 1
ER -