Priming of human oral epithelial cells by interferon-γ to secrete cytokines in response to lipopolysaccharides, lipoteichoic acids and peptidoglycans

A. Uehara, S. Sugawara, H. Takada

Research output: Contribution to journalArticlepeer-review

83 Citations (Scopus)

Abstract

An earlier study reported that human gingival epithelial cells in primary culture and oral epithelial cell lines KB and HSC-2 cells were devoid of membrane CD14 (mCD14) and did not show enhanced production of interleukin (IL)-8 or granulocyte macrophage-colony stimulating factor (GM-CSF) upon stimulation with bacterial cell-surface components such as lipopolysaccharide (LPS), lipoteichoic acid (LTA), peptidoglycan (PGN) and synthetic muramyldipeptide (MDP) even in the presence of serum. The present study demonstrated that after treatment with interferon (IFN)-γ for 3 days, these cells secreted IL-8 and GM-CSF in response to the bacterial components. Treatment with IFN-γ enhanced Toll-like receptor (TLR) 2, TLR4, MD-2 and MyD88 mRNA expression as determined by reverse transcriptase PCR. Anti-TLR2 and anti-TLR4 monoclonal antibodies (MAbs) inhibited the IL-8 production induced by PGN and LTA as well as LPS, respectively, in IFN-γ-primed oral epithelial cells, whereas neither MAb inhibited IL-8 production induced by MDP. These findings suggested that IFN-γ primed oral epithelial cells to produce cytokines upon stimulation with various bacterial components by up-regulation of the TLR system.

Original languageEnglish
Pages (from-to)626-634
Number of pages9
JournalJournal of Medical Microbiology
Volume51
Issue number8
DOIs
Publication statusPublished - 2002

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Fingerprint Dive into the research topics of 'Priming of human oral epithelial cells by interferon-γ to secrete cytokines in response to lipopolysaccharides, lipoteichoic acids and peptidoglycans'. Together they form a unique fingerprint.

Cite this