TY - JOUR
T1 - Primary Structure of Non-Histone Chromosomal Protein HMG2 Revealed by the Nucleotide Sequence
AU - Shirakawa, Hitoshi
AU - Tsuda, Koh Ichiroh
AU - Yoshida, Michiteru
PY - 1990/5/1
Y1 - 1990/5/1
N2 - The isolation and sequencing of a cDNA clone for the entire sequence of pig thymus non-histone protein HMG2 are described. cDNA the size of 1153 nucleotides contains an open reading frame of 627 nucleotides. The 5′-untranslated region of 146 nucleotides is extremely rich in GC residues whereas the 3′-untranslated region of 380 nucleotides is rich in AT residues. The open reading frame encodes 209 amino acids, which contain a unique continuous run of 23 acidic amino acids at the C-terminal. The deduced amino acid sequence is 79% homologous to that of HMG1 protein from the same source which we reported [Tsuda, K., Kikuchi, M, Mori, K., Waga, S., & Yoshida, M. (1988) Biochemistry 27, 6159–6163]. In addition, the hydropathy index profiles of both proteins are very similar, supporting that they have similar structural features. Northern analysis of poly(A+) RNA reveals that a single-sized mRNA codes for HMG2 protein. Southern analysis suggests that the HMG2 coding gene is homogeneous within the pig thymus genome.
AB - The isolation and sequencing of a cDNA clone for the entire sequence of pig thymus non-histone protein HMG2 are described. cDNA the size of 1153 nucleotides contains an open reading frame of 627 nucleotides. The 5′-untranslated region of 146 nucleotides is extremely rich in GC residues whereas the 3′-untranslated region of 380 nucleotides is rich in AT residues. The open reading frame encodes 209 amino acids, which contain a unique continuous run of 23 acidic amino acids at the C-terminal. The deduced amino acid sequence is 79% homologous to that of HMG1 protein from the same source which we reported [Tsuda, K., Kikuchi, M, Mori, K., Waga, S., & Yoshida, M. (1988) Biochemistry 27, 6159–6163]. In addition, the hydropathy index profiles of both proteins are very similar, supporting that they have similar structural features. Northern analysis of poly(A+) RNA reveals that a single-sized mRNA codes for HMG2 protein. Southern analysis suggests that the HMG2 coding gene is homogeneous within the pig thymus genome.
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U2 - 10.1021/bi00470a022
DO - 10.1021/bi00470a022
M3 - Article
C2 - 2350545
AN - SCOPUS:0025285226
VL - 29
SP - 4419
EP - 4423
JO - Biochemistry
JF - Biochemistry
SN - 0006-2960
IS - 18
ER -