Primary structure of human pepsinogen gene

K. Sogawa, Y. Fujii-Kuriyama, Y. Mizukami, Y. Ichihara, K. Takahashi

Research output: Contribution to journalArticlepeer-review

140 Citations (Scopus)

Abstract

A recombinant clone, which covers the pepsinogen gene in a single insert, has been isolated by screening a library of human genomic DNA, using a swine pepsinogen cDNA as a probe. Sequence analysis of coding DNA segments of the clone revealed that the pepsinogen gene occupies approximately 9.4-kilobase pairs of the genomic DNA and is separated into nine exons by eight introns of various lengths. The predicted amino acid sequence of human pepsinogen consists of 373 residues and is 82% homologous with that of swine pepsinogen. In addition, the predicted sequence contained a single sequence of 15 amino acid residues at the NH2 terminus, showing that the protein is synthesized as prepepsinogen. The structure of the gene, in which two homologous sequences including the two active site aspartyl residues of pepsin are present in different coding segments, is in support of the view that the pepsinogen gene evolved by duplication of a shorter ancestral gene.

Original languageEnglish
Pages (from-to)5306-5311
Number of pages6
JournalJournal of Biological Chemistry
Volume258
Issue number8
Publication statusPublished - 1983
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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