Primary structure of human pancreatic α-amylase gene: its comparison with human salivary α-amylase gene

Horii Akira, Emi Mitsuru, Tomita Naohiro, Nishide Takahiro, Ogawa Michio, Mori Takesada, Matsubara Kenichi

Research output: Contribution to journalArticlepeer-review

55 Citations (Scopus)

Abstract

We have determined the entire structure of the human pancreatic α-amylase (Amy2) gene. It is approx. 9 kb long and is separated into ten exons. This gene (amy2) has a structure very similar to that of human salivary α-amylase (Amyl) gene [Nishide et al. Gene 41 (1986a) 299-304] in the nucleotide sequence and the size and location of the exons. The major difference lies in the fact that amyl has one extra exon on the 5' side. Other differences are at the 5' border of exon 1 and the 3' border of exon 10. The close similarity of these two genes, as compared with mouse pancreatic and salivary amylase genes, suggests that during evolution, the divergence into the two amylase genes may have occurred after the divergence of mice and man.

Original languageEnglish
Pages (from-to)57-64
Number of pages8
JournalGene
Volume60
Issue number1
DOIs
Publication statusPublished - 1987

Keywords

  • Recombinant DNA
  • cosmid
  • evolutionary mouse and human divergence
  • exon-intron junctions
  • gene conversion

ASJC Scopus subject areas

  • Genetics

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