Primary structure and transcriptional regulation of rat pepsinogen C gene

T. Ishihara, Y. Ichihara, T. Hayano, I. Katsura, K. Sogawa, Y. Fujii-Kuriyama, K. Takahashi

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    34 Citations (Scopus)

    Abstract

    The entire rat pepsinogen C gene has been isolated from a rat genomic library, using the rat pepsinogen C cDNA as a probe. Southern blot analysis showed that there exists at least two rat pepsinogen C genes. The nucleotide sequences of the coding regions and the 5'- and 3'-flanking regions of one of the rat pepsinogen C genes have been determined. This gene is split into 9 exons interrupted by eight intervening sequences. The 5'-flanking region is similar to that of the human pepsinogen C gene, but only the former had the core sequence of the Sp1 binding site. The amount of transcripts of the rat pepsinogen C genes was found to increase during development, and a similar increase was shown to be induced by injection of hydrocortisone. As a candidate of a factor which regulates the transcription, we found a 25-kDa protein by Southwestern blotting. It binds to a specific site in the 5'-flanking region of the gene only in the presence of Mg2+ ion, and it is present in the nuclear fraction of the gastric mucosa but not of the liver.

    Original languageEnglish
    Pages (from-to)10193-10199
    Number of pages7
    JournalJournal of Biological Chemistry
    Volume264
    Issue number17
    Publication statusPublished - 1989 Jan 1

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology
    • Cell Biology

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