Primary structure and functional expression of the ω-conotoxin-sensitive N-type calcium channel from rabbit brain

Yoshihiko Fujita, Michelle Mynlieff, Robert T. Dirksen, Man Suk Kim, Tetsuhiro Niidome, Junichi Nakai, Thomas Friedrich, Naoyuki Iwabe, Takashi Miyata, Teiichi Furuichi, Daisuke Furutama, Katsuhiko Mikoshiba, Yasuo Mori, Kurt G. Beam

Research output: Contribution to journalArticlepeer-review

203 Citations (Scopus)

Abstract

The complete amino acid sequence of a rabbit brain calcium channel (BIII) has been deduced by cloning and sequencing cDNA. The open reading frame encodes 2339 amino acids, which corresponds to an Mr of 261,167. A phylogenetic tree representing evolutionary relationships indicates that BIII is grouped together with the other rabbit brain calcium channels, BI and BII, into a subfamily t hat is distinct from the dihydropyridine-sensitive L-type subfamily. Transient expression in cultured skeletal muscle myotubes derived from muscular dysgenic mice demonstrates that the BIII channel mediates an ω-conotoxin-sensitive calcium current with kinetics and voltage dependence like those previously reported for whole-cell N-type current. Cell-attached patch recordings, with isotonic barium as the charge carrier, revealed distinct single channels with an average slope conductance of 14.3 pS.

Original languageEnglish
Pages (from-to)585-598
Number of pages14
JournalNeuron
Volume10
Issue number4
DOIs
Publication statusPublished - 1993 Apr
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience(all)

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