Primary structure and functional expression from complementary DNA of a brain calcium channel

Yasuo Mori; Thomas Friedrich; Man Suk Kim; Atsushi Mikami; Junichi Nakai; Peter Ruth; Eva Bosse; Franz Hofmann; Veit Flockerzi; Teiichi Furuichi; Katsuhiko Mikoshiba; Keiji Imoto; Tsutomu Tanabe; Shosaku Numa

doi:10.1038/350398a0

Primary structure and functional expression from complementary DNA of a brain calcium channel

Yasuo Mori, Thomas Friedrich, Man Suk Kim, Atsushi Mikami, Junichi Nakai, Peter Ruth, Eva Bosse, Franz Hofmann, Veit Flockerzi, Teiichi Furuichi, Katsuhiko Mikoshiba, Keiji Imoto, Tsutomu Tanabe, Shosaku Numa

Research output: Contribution to journal › Article › peer-review

706 Citations (Scopus)

Abstract

The primary structure of a voltage-dependent cal-cium channel from rabbit brain has been deduced by cloning and sequencing the complementary DNA. Calcium channel activity expressed from the cDNA is dramatically increased by coexpression of the α₂ and β subunits, known to be associated with the dihydropyridine receptor. This channel is a high voltage-activated calcium channel that is insensitive both to nifedipine and to ω-conotoxin. We suggest that it is expressed predominantly in cerebellar Purkinje cells and granule cells.

Original language	English
Pages (from-to)	398-402
Number of pages	5
Journal	Nature
Volume	350
Issue number	6317
DOIs	https://doi.org/10.1038/350398a0
Publication status	Published - 1991
Externally published	Yes

ASJC Scopus subject areas

General

Access to Document

10.1038/350398a0

Cite this

@article{9572086f5fbd4e9ea3bd65e6abb507f8,

title = "Primary structure and functional expression from complementary DNA of a brain calcium channel",

abstract = "The primary structure of a voltage-dependent cal-cium channel from rabbit brain has been deduced by cloning and sequencing the complementary DNA. Calcium channel activity expressed from the cDNA is dramatically increased by coexpression of the α2 and β subunits, known to be associated with the dihydropyridine receptor. This channel is a high voltage-activated calcium channel that is insensitive both to nifedipine and to ω-conotoxin. We suggest that it is expressed predominantly in cerebellar Purkinje cells and granule cells.",

author = "Yasuo Mori and Thomas Friedrich and Kim, {Man Suk} and Atsushi Mikami and Junichi Nakai and Peter Ruth and Eva Bosse and Franz Hofmann and Veit Flockerzi and Teiichi Furuichi and Katsuhiko Mikoshiba and Keiji Imoto and Tsutomu Tanabe and Shosaku Numa",

year = "1991",

doi = "10.1038/350398a0",

language = "English",

volume = "350",

pages = "398--402",

journal = "Nature",

issn = "0028-0836",

publisher = "Nature Publishing Group",

number = "6317",

}

TY - JOUR

T1 - Primary structure and functional expression from complementary DNA of a brain calcium channel

AU - Mori, Yasuo

AU - Friedrich, Thomas

AU - Kim, Man Suk

AU - Mikami, Atsushi

AU - Nakai, Junichi

AU - Ruth, Peter

AU - Bosse, Eva

AU - Hofmann, Franz

AU - Flockerzi, Veit

AU - Furuichi, Teiichi

AU - Mikoshiba, Katsuhiko

AU - Imoto, Keiji

AU - Tanabe, Tsutomu

AU - Numa, Shosaku

PY - 1991

Y1 - 1991

N2 - The primary structure of a voltage-dependent cal-cium channel from rabbit brain has been deduced by cloning and sequencing the complementary DNA. Calcium channel activity expressed from the cDNA is dramatically increased by coexpression of the α2 and β subunits, known to be associated with the dihydropyridine receptor. This channel is a high voltage-activated calcium channel that is insensitive both to nifedipine and to ω-conotoxin. We suggest that it is expressed predominantly in cerebellar Purkinje cells and granule cells.

AB - The primary structure of a voltage-dependent cal-cium channel from rabbit brain has been deduced by cloning and sequencing the complementary DNA. Calcium channel activity expressed from the cDNA is dramatically increased by coexpression of the α2 and β subunits, known to be associated with the dihydropyridine receptor. This channel is a high voltage-activated calcium channel that is insensitive both to nifedipine and to ω-conotoxin. We suggest that it is expressed predominantly in cerebellar Purkinje cells and granule cells.

UR - http://www.scopus.com/inward/record.url?scp=0025775195&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025775195&partnerID=8YFLogxK

U2 - 10.1038/350398a0

DO - 10.1038/350398a0

M3 - Article

C2 - 1849233

AN - SCOPUS:0025775195

SN - 0028-0836

VL - 350

SP - 398

EP - 402

JO - Nature

JF - Nature

IS - 6317

ER -

Primary structure and functional expression from complementary DNA of a brain calcium channel

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this