Primary structure and functional expression from cDN A of the cardiac ryanodine receptor/calcium release channel

Junichi Nakai, Toshiaki Imagawa, Yasuhiro Hakamata, Munekazu Shigekawa, Hiroshi Takeshima, Shosaku Numa

Research output: Contribution to journalArticlepeer-review

277 Citations (Scopus)

Abstract

The sequence of 4968 (or 4976 with an insertion) amino acids composing the ryanodine receptor from rabbit cardiac sarcoplasmic reticulum has been deduced by cloning and sequencing the cDNA. This protein is homologous in amino acid sequence and shares characteristic structural features with the skeletal muscle ryanodine receptor. Xenopus oocytes injected with mRNA derived from the cardiac ryanodine receptor cDNA exhibit Ca2+-dependent Cl- current in response to caffeine, which indicates the formation of functional calcium release channels. RNA blot hybridization analysis with a probe specific for the cardiac ryanodine receptor mRNA shows that the stomach and brain contain a hybridizable RNA species with a size similar to that of the cardiac mRNA. This result, in conjunction with cloning and analysis of partial cDNA sequences, suggests that the brain contains a cardiac type of ryanodine receptor mRNA.

Original languageEnglish
Pages (from-to)169-177
Number of pages9
JournalFEBS Letters
Volume271
Issue number1-2
DOIs
Publication statusPublished - 1990 Oct 1

Keywords

  • Calcium release channel
  • RNA blot hybridization analysis
  • Rabbit heart
  • Ryanodine receptor
  • cDNA cloning
  • cDNA expression

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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