Abstract
We previously demonstrated that in murine lethal listeriosis, death is mainly due to massive liver necrosis. In the present study we found that in vivo depletion of αβ T cells by administration of anti-TCR β mAb (H57-597) protected recipient mice from acute mortality and converted lethal listeriosis to sublethal infection. Furthermore, our findings suggested that γδ T cells were not involved in the liver necrosis in this condition. After depletion of αβ T cells, the number of bacteria decreased gradually to the limit for their detection (102 CFU) in 4 wks. Depletion of both αβ T cells and γδ T cells by administration of anti-TCR β mAb (H57-597) and anti-TCR δ mAb (3A10) resulted in increased multiplication and persistent presence of bacteria in the liver and spleen. These findings suggest that γδ T cells play a significant protective role during infection in mice depleted of αβ T cells. In these mice, γδ T cells appeared with a peak on day 13 in the liver and on day 20 in the lymph nodes. No increase of γδ T cells in the spleen was observed throughout the course of infection.
Original language | English |
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Pages (from-to) | 71-83 |
Number of pages | 13 |
Journal | Immunobiology |
Volume | 193 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1995 Jan 1 |
Externally published | Yes |
Keywords
- ALT
- CFU
- H-E
- TSA
- TSB
- alanine amino transferase
- anti-
- colony forming units
- hematoxylin-eosin
- trypticase soy agar
- trypticase soy broth
- α
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Hematology