Pretreatment with rumen fluid improves methane production in the anaerobic digestion of paper sludge

Shuhei Takizawa, Yasunori Baba, Chika Tada, Yasuhiro Fukuda, Yutaka Nakai

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


Because paper sludge discharged from the waste paper recycling process contains high levels of lignin and ash, it is not hydrolyzed effectively during anaerobic digestion. In this study, we investigated the effects of pretreatment with rumen fluid on paper sludge and on the methane fermentation process. Paper sludge was pretreated with rumen fluid at 37 °C for 6 h. Following pretreatment, 4.5% of the total solids in paper sludge were degraded and converted, and the dissolved chemical oxygen demand and volatile fatty acid concentration increased. Batch methane fermentation was conducted at 37 °C for 20 days. During methane fermentation, the degradation and hydrolysis of paper sludge were enhanced by pretreatment with rumen fluid. The amounts of total methane production from pretreated paper sludge (excluding methane generated from rumen fluid), rumen fluid and untreated paper sludge were 650.4, 819.9 and 190.8 ml, respectively. The volume of methane gas produced from pretreated paper sludge was 3.4 times larger than that from untreated paper sludge. These results indicate that pretreatment with rumen fluid enhances methane production from paper sludge.

Original languageEnglish
Pages (from-to)379-384
Number of pages6
JournalWaste Management
Publication statusPublished - 2018 Aug


  • Anaerobic digestion
  • Methane
  • Paper sludge
  • Pretreatment
  • Rumen fluid

ASJC Scopus subject areas

  • Waste Management and Disposal


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