TY - JOUR
T1 - Pretreatment of lignocellulosic biomass with cattle rumen fluid for methane production
T2 - Fate of added rumen microbes and indigenous microbes of methane seed sludge
AU - Baba, Yasunori
AU - Matsuki, Yu
AU - Takizawa, Shuhei
AU - Suyama, Yoshihisa
AU - Tada, Chika
AU - Fukuda, Yasuhiro
AU - Saito, Masanori
AU - Nakai, Yutaka
N1 - Funding Information:
This study was supported in part by the Japan Society for the Promotion of Science (Grant-in-Aid for JSPS Research Fellows Grant Number 14J08197, Grant-in-Aid for Scientific Research (A) Grant Number 17H01512, and Early-Career Scientists Grant Number 18K18219) and the Next-generation Energies for Tohoku Recovery (NET) Project. The authors declare that they have no competing interests. The authors wish to thank Mr. Kazuya Sato and Ms. Rie Yamamoto, the Field Science Center, Tohoku University, for their technical support during the collection of rumen contents. The authors also thank Assistant Professor Michiaki Omura, Ms. Yukie Ogushi, and Ms. Takako Sasaki for their help with the collection of rapeseed.
Publisher Copyright:
© 2019, Japanese Society of Microbial Ecology. All rights reserved.
PY - 2019
Y1 - 2019
N2 - The pretreatment of lignocellulosic substrates with cattle rumen fluid was successfully developed to increase methane production. In the present study, a 16S rRNA gene-targeted amplicon sequencing approach using the MiSeq platform was applied to elucidate the effects of the rumen fluid treatment on the microbial community structure in laboratory-scale batch methane fermenters. Methane production in fermenters fed rumen fluid-treated rapeseed (2,077.3 mL CH4 reactor–1 for a 6-h treatment) was markedly higher than that in fermenters fed untreated rapeseed (1,325.8 mL CH4 reactor–1). Microbial community profiling showed that the relative abundance of known lignocellulose-degrading bacteria corresponded to lignocellulose-degrading enzymatic activities. Some dominant indigenous cellulolytic and hemicellulolytic bacteria in seed sludge (e.g., Cellulosilyticum lentocellum and Ruminococcus flavefaciens) and rumen fluid (e.g., Butyrivibrio fibrisolvens and Prevotella ruminicola) became undetectable or markedly decreased in abundance in the fermenters fed rumen fluid-treated rapeseed, whereas some bacteria derived from seed sludge (e.g., Ruminofilibacter xylanolyticum) and rumen fluid (e.g., R. albus) remained detectable until the completion of methane production. Thus, several lignocellulose-degrading bacteria associated with rumen fluid proliferated in the fermenters, and may play an important role in the degradation of lignocellulosic compounds in the fermenter.
AB - The pretreatment of lignocellulosic substrates with cattle rumen fluid was successfully developed to increase methane production. In the present study, a 16S rRNA gene-targeted amplicon sequencing approach using the MiSeq platform was applied to elucidate the effects of the rumen fluid treatment on the microbial community structure in laboratory-scale batch methane fermenters. Methane production in fermenters fed rumen fluid-treated rapeseed (2,077.3 mL CH4 reactor–1 for a 6-h treatment) was markedly higher than that in fermenters fed untreated rapeseed (1,325.8 mL CH4 reactor–1). Microbial community profiling showed that the relative abundance of known lignocellulose-degrading bacteria corresponded to lignocellulose-degrading enzymatic activities. Some dominant indigenous cellulolytic and hemicellulolytic bacteria in seed sludge (e.g., Cellulosilyticum lentocellum and Ruminococcus flavefaciens) and rumen fluid (e.g., Butyrivibrio fibrisolvens and Prevotella ruminicola) became undetectable or markedly decreased in abundance in the fermenters fed rumen fluid-treated rapeseed, whereas some bacteria derived from seed sludge (e.g., Ruminofilibacter xylanolyticum) and rumen fluid (e.g., R. albus) remained detectable until the completion of methane production. Thus, several lignocellulose-degrading bacteria associated with rumen fluid proliferated in the fermenters, and may play an important role in the degradation of lignocellulosic compounds in the fermenter.
KW - Bioaugmentation
KW - Lignocellulose
KW - Methane fermentation
KW - MiSeq
KW - Rumen
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U2 - 10.1264/jsme2.ME19113
DO - 10.1264/jsme2.ME19113
M3 - Article
C2 - 31748428
AN - SCOPUS:85077436125
VL - 34
SP - 421
EP - 428
JO - Microbes and Environments
JF - Microbes and Environments
SN - 1342-6311
IS - 4
ER -