Preservation of rumen fluid for the pretreatment of waste paper to improve methane production

Shuhei Takizawa, Yasunori Baba, Chika Tada, Yasuhiro Fukuda, Yutaka Nakai

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


It is necessary to preserve rumen fluid for transport from slaughterhouses to the pretreatment facilities for use in treating lignocellulosic biomass. In this study, we investigated how the preservation of rumen fluid at various temperatures affects its use in hydrolysis of waste paper. Rumen fluid was preserved anaerobically at 4, 20, and 35 °C for 7 days. The number of protozoa and fibrolytic enzyme activity after preservation at 4 °C were significantly higher than that after preservation at either 20 or 35 °C. Waste paper was subsequently treated with preserved rumen fluid at 37 °C for 48 h. Preservation at 20 °C remarkedly decreased the hydrolysis of waste paper. Xylanase activity in rumen fluid preserved at 35 °C increased during the treatment, which enhanced the solubilization of waste paper as comparable to the control and preservation at 4 °C. Pretreatment of waste paper with rumen fluid preserved at 4 °C showed that the fluid retained high fibrolytic activity, and reduced the loss of organic carbon as substrate for methanogens. Our results suggest that preservation of rumen fluid at 4 °C is most suitable for efficient pretreatment and methane fermentation of waste paper.

Original languageEnglish
Pages (from-to)672-678
Number of pages7
JournalWaste Management
Publication statusPublished - 2019 Mar 15


  • Enzyme activity
  • Hydrolysis
  • Rumen fluid
  • Waste paper

ASJC Scopus subject areas

  • Waste Management and Disposal


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